AdipoGen Life Sciences

anti-GPX3, pAb

CHF 190.00

In stock

AG-25A-0073-C100100 µgCHF 190.00

Figure 1: Western blot analysis using anti-GPX3 (mouse), pAb (Prod. No. AG-25A-0073) at 1:2'000 dilution.
1: Mouse GPX3 (FLAG^®-tagged).
2: Human GPX3 (FLAG^®-tagged)
3: Mouse serum #1 (Balb/c, 2μl)
4: Mouse serum #2 (ICR, 2μl)

Specifications / Handling

More Information
Product Details
Synonyms	Glutathione Peroxidase 3
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant mouse GPX3.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Mouse
Specificity	Recognizes mouse GPX3. Detects a band of ~20-27kDa by Western blot. Weakly cross-reacts with human GPX3.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Glutathione peroxidase 3 (GPX3; GSHPX3) is a selenium-dependent extracellular enzyme, protecting cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxides. GPX3 is involved in inflammatory bowel disease, in asthma and in diabetes. The activity of GPX3 is significantly reduced in the plasma and tissue of cancer patients. Recently, GPX3 was suggested to serve as a molecular marker for the diagnosis of clear cell type ovarian adenocarcinoma.

Product References

Glutathione peroxidase 3 mediates the antioxidant effect of peroxisome proliferator-activated receptor gamma in human skeletal muscle cells: S.S. Chung, et al.; Mol. Cell Biol. 29, 20 (2009)
Isocitrate dehydrogenase 2 protects mice from high-fat diet-induced metabolic stress by limiting oxidative damage to the mitochondria from brown adipose tissue: J.H. Lee, et al.; Exp. Mol. Med. 52, 238 (2020)

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