AdipoGen Life Sciences

anti-Sirtuin 5 (human), pAb

CHF 190.00

In stock

AG-25A-0100-C100100 µgCHF 190.00

Figure 1: Western blot analysis using anti-Sirtuin 5 (human), pAb (Prod. No. AG-25A-0100) at 1:2'000 dilution.
1: Human Sirtuin 5 (intact form) (His-tagged).
2: Human Sirtuin 1 (His-tagged).
3: Human Sirtuin 2 (His-tagged).
4: Human Sirtuin 6 (His-tagged).
5: Mouse IDO (His-tagged) (negative control).

Specifications / Handling

More Information
Product Details
Synonyms	SIRT5; NAD-dependent Deacetylase Sirtuin-5; SIR2-like Protein 5; SIR2L5
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human sirtuin 5.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes human sirtuin 5 (intact form). Detects a band of ~37kDa by Western blot.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Sirtuin 5 is a NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during fasting. Can deacetylate cytochrome c and a number of other proteins (in vitro).

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