AdipoGen Life Sciences

anti-CTRP1 (human), pAb

CHF 190.00

In stock

AG-25A-0114-C100100 µgCHF 190.00

Figure 1: Immunohistochemical staining of CTRP1 using anti-CTRP1 (human), pAb (Prod. No. AG-25A-0114) in human testis tissue (15µg/ml).
This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Figure 2: Western blot analysis of recombinant human CTRPs using anti-CTRP1 (human), pAb (Prod. No. AG-25A-0114) at 1:4,000 dilution.
1: Recombinant human CTRP1 protein (His-tagged).
2: Unrelated recombinant protein (His-tagged).

Figure 3: Immunoprecipitation (IP) analysis of the cell lysates from HEK293 cells transfected with empty vector or a panel of the FLAG-tagged CTRP family (full-length) followed by immunoblot analysis using anti-CTRP1 (human), pAb (Prod. No. AG-25A-0114) at 1:4,000 dilution. The immunoblot (IB) analysis demonstrates the specificity of the anti-CTRP1 (human), pAb (Prod. No. AG-25A-0114) antibody.
3: hCTRP1-FLAG (full-length) transfected HEK293 cell lysates.
4: hCTRP2-FLAG (full-length) transfected HEK293 cell lysates.
5: hCTRP3-FLAG (full-length) transfected HEK293 cell lysates.
6: hCTRP5-FLAG (full-length) transfected HEK293 cell lysates.
7: hCTRP6-FLAG (full-length) transfected HEK293 cell lysates.
8: hCTRP7-FLAG (full-length) transfected HEK293 cell lysates.
9: hCTRP9-FLAG (full-length) transfected HEK293 cell lysates.
10: hCTRP10-FLAG (full-length) transfected HEK293 cell lysates.
11: hCTRP11-FLAG (full-length) transfected HEK293 cell lysates.
12: hCTRP13-FLAG (full-length) transfected HEK293 cell lysates.
13: Empty vector transfected HEK293 cell lysates.

Specifications / Handling

More Information
Product Details
Synonyms	Complement C1q Tumor Necrosis Factor-related Protein 1; C1QTNF1; G Protein-coupled Receptor-interacting Protein; GIP
Product Type	Polyclonal Antibody
Properties
Source/Host	Guinea pig
Immunogen/Antigen	Recombinant human CTRP1.
Application	ELISA: (direct and indirect: 1:2’000-1:5’000) Immunohistochemistry: (15μg/ml) Immunoprecipitation: (1:500-1:2’000) Western Blot: (1:2’000-1:5’000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. Note: Tested on recombinant proteins and/or target-protein transfected cell lines in ELISA, Western Blot and/or FACS.
Crossreactivity	Human
Specificity	Recognizes a N-terminal region of CTRP1 full-length protein. Detects bands of ~17kDa and ~36kDa by Western blot. This antibody is CTRP1-specific.
Purity Detail	Protein A-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

CTRP1 (Complement C1q tumor necrosis factor-related protein 1; C1QTNF1) belongs to a highly conserved family of adiponectin paralogs. Serum levels of CTRP1 are significantly higher in obese/diabetic animal models and in hypertensive patients compared to normal controls. CTRP1 may be a new molecular link between obesity and hypertension.

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