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AdipoGen Life Sciences
anti-MDA5 (mouse), pAb (AL180)
440
CHF
CHF 440.00
In stock
AG-25B-0001V-C100100 µgCHF 440.00
Figure 1: Western blot analysis anti-MDA5 (mouse), pAb (AL180) (Prod. No. AG-25B-0001V) detecting the cleavage of endogenous mouse MDA5 as an indicator of apoptosis. Method: Cleavage of endogenous Helicard in murine A20 B lymphomas undergoing Fas-mediated apoptosis. A20 cells, either sensitive (S) or resistant (R) to FasL, were treated with 200 ng/ml of FasL for 4 hours and harvested at the indicated times. The cells were lysed and the cell extracts resolved by SDS-PAGE under reducing conditions. After transfer to nitrocellulose the membrane was incubated with anti-MDA5 (mouse), pAb (AL180) at a dilution of 1:1'000 for 2 hours. Detection of MDA5 was performed using a peroxidase-conjugated antibody to rabbit Ig (Jackson Lab.) followed by a chemiluminescence detection system. Note: In non-stimulated A20S cells MDA5 has a molecular weight of 140kDa. Upon FasL-induced activation, cleavage products of 120, 41/45, 30 and 28kDa can be detected.
| Product Details | |
|---|---|
| Synonyms | Helicard; Melanoma Differentiation-associated Gene 5 |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | Recombinant mouse MDA5 (aa 2-208). |
| Application |
Immunohistochemistry: (frozen sections) *Note: We recommend a starting dilution of 1:1'000 and to optimize the dilution depending on your cells/tissues. |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse MDA5. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg /ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
This purified antiserum is replacing the product AG-25B-0001 MDA5 pAb (AL180) (neat antiserum) with same specifications. |
| Accession Number | Q8R5F7 |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. MDA5, upon interaction with intracellular dsRNA (produced during viral replication), triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-κB, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-β and RANTES (CCL5). MDA5 is widely expressed at low levels. The expression is detected at higher levels in placenta, pancreas and spleen and barely in brain, testis and lung. Genetic variation in MDA5 is associated with diabetes mellitus insulin-dependent type 19 (IDDM19). A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy.
Product References
- Overexpression of Helicard, a CARD-Containing Helicase Cleaved during Apoptosis, Accelerates DNA Degradation: M. Kovacsovics, et al.; Curr. Biol. 12, 838 (2002)
- Distinct and complementary functions of MDA5 and TLR3 in poly(I:C)-mediated activation of mouse NK cells: S. McCartney, et al.; J. Exp. Med. 206, 2967 (2009)
- HOIL1 Is Essential for the Induction of Type I and III Interferons by MDA5 and Regulates Persistent Murine Norovirus Infection: D.A. MacDuff, et al.; J. Virol. 92, e01368 (2018)
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