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AdipoGen Life Sciences
anti-CARMA1, pAb (AL220)
430
CHF
CHF 430.00
In stock
AG-25B-0004V-C100100 µgCHF 430.00
Figure 1: Immunocytochemistry analysis of CARMA1 using anti-CARMA1, pAb (AL220) (Prod. No. AG-25B-0004V).
Method: Human T cells (clone 6396p5.1.2) were incubated with anti-CD3, mAb for 20 min. on ice, followed by incubation with secondary goat anti-mouse for 15 min. at 4°C or 37°C. Cells were stained for CD3 (green), phosphotyrosine (red) and CARMA1 (1:200 dilution; blue) and analyzed by confocal microscopy.
Method: Human T cells (clone 6396p5.1.2) were incubated with anti-CD3, mAb for 20 min. on ice, followed by incubation with secondary goat anti-mouse for 15 min. at 4°C or 37°C. Cells were stained for CD3 (green), phosphotyrosine (red) and CARMA1 (1:200 dilution; blue) and analyzed by confocal microscopy.
| Product Details | |
|---|---|
| Synonyms | CARD11; Bimp3; Caspase Recruitment Domain-containing Protein 11; CARD-containing MAGUK Protein 1 |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | N-terminal human CARMA1 (aa 1-221 containing the CARD domain). |
| Application |
Immunocytochemistry:(1:200) |
| Crossreactivity |
Human Mouse |
| Specificity |
Recognizes human and mouse CARMA1. Detects a band of ~130Dka by Western blot. |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
CARMA1 is involved in the costimulatory signal essential for T cell receptor (TCR)-mediated T cell activation. Its binding to DPP4 induces T cell proliferation and NF-κB activation in a T cell receptor/CD3-dependent manner. CARMA1 activates NF-κB via Bcl10 and IKK.
Product References
- CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappaB activation: O. Gaide, et al.; Nat. Immunol. 3, 836 (2002)
- MALT1 and the API2-MALT1 fusion act between CD40 and IKK and confer NF-κB-dependent proliferative advantage and resistance against FAS-induced cell death in B cells: L. Ho, et al.; Blood 105, 2891 (2005)
- Phosphorylation of the CARMA1 Linker Controls NF-κB Activation: K. Sommer, et al.; Immunity 23, 561 (2005)
- Overexpression of Caspase Recruitment Domain (CARD) Membrane-Associated Guanylate Kinase 1 (CARMA1) and CARD9 in Primary Gastric B-Cell Lymphoma: S. Nakamura, et al.; Cancer 104, 1885 (2005)
- Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1: R.B. Medeiros, et al.; Science 316, 754 (2007)
- Bcl10 Controls TCR- and FcγR-Induced Actin Polymerization: D. Rueda, et al.; J. Immunol. 178, 4373 (2007)
- The proteolytic activity of the paracaspase MALT1 is key in T cell activation: F. Rebeaud, et al.; Nat. Immunol. 9, 272 (2008)
- MAGUK-Controlled Ubiquitination of CARMA1 Modulates Lymphocyte NF-κB Activity: M.E. Moreno-Garcia, et al.; Mol. Cell Biol. 30, 922 (2010)
- Interaction of calmodulin with Bcl10 modulates NF-κB activation: S. Edin, et al.; Mol. Immunol. 47, 2057 (2010)
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