AdipoGen Life Sciences

anti-PIDD, pAb (AL233)

Name: anti-PIDD, pAb (AL233)
Brand: AdipoGen Life Sciences
SKU: AG-25B-0015
Price: 270.00 CHF
Availability: InStock

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AG-25B-0015-C05050 µgCHF 270.00

Figure 1: Western blot analysis of human HCT116 cells using anti-PIDD, pAb (AL233) (Prod. No. AG-25B-0015). HCT116 cells of WT FANCI and -/- FANCI genotypes and stably expressing shp53 were treated with Gö6976 and MMC (1mM each), harvested 24h post-MMC, and were analyzed by western blot.

Specifications / Handling

More Information
Product Details
Synonyms	p53-induced Protein with a Death Domain; Leucine-rich Repeat and Death Domain-containing Protein; Lrdd
Product Type	Polyclonal Antibody
Properties
Source/Host	Rabbit
Immunogen/Antigen	Recombinant human PIDD (Death Domain: aa 776-910).
Application	Immunohistochemistry: (paraffin sections) Immunoprecipitation: (1:100) Western Blot: (1:500)
Crossreactivity	Human Mouse
Specificity	Recognizes human and the native form of mouse PIDD. Detects bands of ~90kDa (Full Length FL-PIDD) and ~50kDa (cleaved protein) by Western blot.
Purity Detail	Epitope-affinity purified.
Concentration	1 mg/ml
Formulation	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control	Rabbit IgG
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
Safety Data Sheet	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

PIDD promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. It associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-κ-B. It associates with caspase-2 and RAIDD, and induces activation of caspase-2 which is an important regulator in the apoptotic pathways.

Product-specific References

The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel & J. Tschopp; Science 304, 843 (2004)
PIDD mediates NF-kB activation in Response to DNA damage: Janssens et al.; Cell 123, 1079 (2005)
Heat shock induces apoptosis independently of any known initiator caspase-activating complex: R.S. Milleron & S.B. Bratton; J. Biol. Chem. 281, 16991 (2006)
An Inhibitor of PIDDosome Formation: R. Thompson, et al.; Mol. Cell 58, 767 (2015)
NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus: K. Ando, et al.; J. Cell Biol. 216, 1795 (2017)
FANCI functions as a repair/apoptosis switch in response to DNA crosslinks: R.B. Shah, et al.; Dev. Cell 56, 1 (2021)

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