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AdipoGen Life Sciences
anti-PIDD, pAb (AL233)
As low as
270
CHF
CHF 270.00
In stock
Only %1 left
AG-25B-0015-C05050 µgCHF 270.00
Figure 1: Western blot analysis of human HCT116 cells using anti-PIDD, pAb (AL233) (Prod. No. AG-25B-0015). HCT116 cells of WT FANCI and -/- FANCI genotypes and stably expressing shp53 were treated with Gö6976 and MMC (1mM each), harvested 24h post-MMC, and were analyzed by western blot.
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | p53-induced Protein with a Death Domain; Leucine-rich Repeat and Death Domain-containing Protein; Lrdd |
| Product Type | Polyclonal Antibody |
| Properties | |
| Source/Host | Rabbit |
| Immunogen/Antigen | Recombinant human PIDD (Death Domain: aa 776-910). |
| Application |
Immunohistochemistry: (paraffin sections) |
| Crossreactivity |
Human Mouse |
| Specificity |
Recognizes human and the native form of mouse PIDD. Detects bands of ~90kDa (Full Length FL-PIDD) and ~50kDa (cleaved protein) by Western blot. |
| Purity Detail | Epitope-affinity purified. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
PIDD promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. It associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-κ-B. It associates with caspase-2 and RAIDD, and induces activation of caspase-2 which is an important regulator in the apoptotic pathways.
Product-specific References
- The PIDDosome, a protein complex implicated in activation of caspase-2 in response to genotoxic stress: A. Tinel & J. Tschopp; Science 304, 843 (2004)
- PIDD mediates NF-kB activation in Response to DNA damage: Janssens et al.; Cell 123, 1079 (2005)
- Heat shock induces apoptosis independently of any known initiator caspase-activating complex: R.S. Milleron & S.B. Bratton; J. Biol. Chem. 281, 16991 (2006)
- An Inhibitor of PIDDosome Formation: R. Thompson, et al.; Mol. Cell 58, 767 (2015)
- NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus: K. Ando, et al.; J. Cell Biol. 216, 1795 (2017)
- FANCI functions as a repair/apoptosis switch in response to DNA crosslinks: R.B. Shah, et al.; Dev. Cell 56, 1 (2021)
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