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AdipoGen Life Sciences
anti-PSD-95 (palmitoylated), mAb (rec.) (PF11)
455
CHF
CHF 455.00
In stock
AG-27B-0021-C100100 µgCHF 455.00
Figure 1: Palmitoylated PSD95 is detected by immunocytochemistry using anti-PSD-95 (palmitoylated), mAb (rec.) (PF11) (Prod. No AG-27B-0021).
Method: HeLa cells were cotransfected with DHHC2 (palmitoylating enzyme) + PSD95-GFP (A) or DHHC2 (palmitoylating enzyme) alone (B). Cells in "B" were fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-PSD-95 (palmitoylated), mAb (rec.) (PF11) (1µg/ml in PBS-BSA-Saponin). After incubation for 30min at RT and several washes in PBS, cells are treated with a goat anti-human (ATTO488) antibody in PBS-BSA-Saponin for 30min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI. Merge image is shown at the right. Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.
Method: HeLa cells were cotransfected with DHHC2 (palmitoylating enzyme) + PSD95-GFP (A) or DHHC2 (palmitoylating enzyme) alone (B). Cells in "B" were fixed with paraformaldehyde (3%), permeablized in PBS+ BSA 0.2 % + Saponin 0.05 % and incubated with anti-PSD-95 (palmitoylated), mAb (rec.) (PF11) (1µg/ml in PBS-BSA-Saponin). After incubation for 30min at RT and several washes in PBS, cells are treated with a goat anti-human (ATTO488) antibody in PBS-BSA-Saponin for 30min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI. Merge image is shown at the right. Picture courtesy of Dr. Moutel, Dr. Franck Perez Lab, Curie Institute, Paris.
Figure 2: Structure of the recombinant antibody anti-PSD-95 (palmitoylated), mAb (rec.) (PF11) (AG-27B-0021). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.
| Product Details | |
|---|---|
| Synonyms | PSD95; Disks Large Homolog 4; DLG4; Postsynaptic Density Protein 95; Synapse-associated Protein 90; SAP90 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | PF11 |
| Isotype | Human IgG2 |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Palmitoylated PSD-95. |
| Application |
Immunocytochemistry: 1/1000 dilution (fixed with methanol) |
| Crossreactivity |
Human Mouse Rat |
| Specificity |
Recognizes human, mouse and rat palmitoylated PSD-95. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 0.5 mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
anti-PSD95 Palmitoylated, mAb (rec.) (PF11) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
PSD-95 is a member of proteins located at a specialized postsynaptic membrane region, called the postsynaptic density (PSD). PSD-95 is the most abundant scaffold protein specifically enriched in the PSD. It contains three PDZ domains, an SH3 domain and a guanylate kinase (GuK)-like domain. Through its PDZ domains, PSD-95 assembles various synaptic components at the PSD including intracellular signaling molecules (e.g. SynGAP and kalirin-7), ion channels (e.g. stargazin/AMPA receptors [AMPARs] and NMDA receptors) and cell adhesion molecules (e.g. neuroligin). PSD-95 plays a primary role in synaptic development and maturation and is regulated by palmitoylation at its N-terminal cysteine residues leading to its postsynaptic targeting. Palmitoylation of PSD-95 is triggered by DHHC2, a plasma membrane-inserted palmitoylating enzyme. Palmitoylated PSD-95 is almost exclusively localized at excitatory synapses in neurons.
Product References
- Local palmitoylation cycles define activity-regulated postsynaptic subdomains: Y. Fukata, et al.; J. Cell Biol. 202, 145 (2013)
- Palmitoylation regulates glutamate receptor distributions in postsynaptic densities through control of PSD95 conformation and orientation: O. Jeyifous, et al.; PNAS 113, E8482 (2016)
- Ca2+/Calmodulin Binding to PSD-95 Downregulates Its Palmitoylation and AMPARs in Long-Term Depression: D. Chowdhury & J.W. Hell; Front. Synaptic Neurosci. 11, 6 (2019)
- Recombinant Antibodies in Basic Neuroscience Research: J.S. Trimmer; Curr. Prot. in Neurosci. 94, e106 (2020)
- Engineering paralog-specific PSD-95 recombinant binders as minimally interfering multimodal probes for advanced imaging techniques: C. Rimbault, et al.; eLife 13, e69620 (2024)
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