anti-RhoA/B (ADP-ribosylated) (human), mAb (rec.) (VIF140-A1)

Specifications / Handling

More Information
Product Details
Synonyms	Ras Homologous
Product Type	Recombinant Antibody
Properties
Clone	VIF140-A1
Isotype	Human IgG1
Source/Host	Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen	Recombinant ADP-ribosylated human RhoA.
Application	ELISA Immunocytochemistry Western Blot
Crossreactivity	Human
Specificity	Recognizes ADP-ribosylated human RhoA and RhoB, but not RhoC. Does not recognize unmodified non-ADP-ribosylated RhoA or glycosylated RhoA.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein A purified from animal component-free supernatant.
Concentration	1 mg/ml
Formulation	Liquid. In PBS.
Isotype Negative Control	Human IgG1 Isotype Control
Other Product Data	This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Accession Number	P61586, P62745
Declaration	Manufactured by Abcalis
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 1 week when stored at +4°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

The Ras homologous (Rho) protein family of GTPases (RhoA, RhoB and RhoC) are the members of the Ras superfamily and regulate cellular processes such as cell migration, proliferation, polarization, adhesion, gene transcription and cytoskeletal structure. Rho GTPases function as molecular switches that cycle between GTP-bound (active state) and GDP-bound (inactive state) forms.

Product References

Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody: A. Rohrbeck, et al.; Toxins 8, 100 (2016)

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