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AdipoGen Life Sciences
anti-Crf2 (Aspergillus fumigatus), mAb (rec.) (MS112-IIA1)
330
CHF
CHF 330.00
In stock
AG-27B-6318-C05050 µgCHF 330.00
| Product Details | |
|---|---|
| Synonyms | Chitin Ring Formation 2; Crh-like Protein 2 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | MS112-IIA1 |
| Isotype | Human IgG1 |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Recombinant Crf2 (Aspergillus fumigatus) containing a C-terminal His-tag. |
| Application |
FUNC: Neutralizes the enzymatic activity of Crf2 in vitro. [Reference 2] |
| Crossreactivity | Fungus |
| Specificity |
Recognizes Crf2 from Aspergillus fumigatus. Possible cross-reactivity with Crf1, Crf2 and Asp f9 [Reference 2]. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from animal component-free supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Accession Number | Q4WI46 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 1 week when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Aspergillus fumigatus is a common airborne fungal pathogen for humans. It frequently causes an invasive aspergillosis (IA) in immunocompromised patients with poor prognosis. Potent antifungal drugs are very expensive and cause serious adverse effects. Their correct application requires an early and specific diagnosis of IA. Encoded by CRF1 gene, the Crf protein family contains three protein variants: Crf2 protein, amino acids (333 aa) long, Asp f9 protein (292 aa), first identified as an allergen using serum from Aspergillus-allergic patients and Crf1 (395 aa, also named Crh5). The glycosyol hydrolase/glycosidase Crf2 catalyzes the hydrolysis of glycosidic bonds in complex sugars and is located in the cell wall of the growing hyphae. Crf2 may be involved in the remodeling of the cell wall as well, and therefore in the growth and the virulence of the fungus.
Product References
- Identification of a putative Crf splice variant and generation of recombinant antibodies for the specific detection of Aspergillus fumigatus: M. Schutte, et al.; PLoS One 4, e6625 (2009)
- Targeting Aspergillus fumigatus Crf Transglycosylases With Neutralizing Antibody Is Relevant but Not Sufficient to Erase Fungal Burden in a Neutropenic Rat Model: D. Chauvin, et al.; Front. Microbiol. 10, 600 (2019)
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