Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
anti-Hex1 (Neurospora crassa), mAb (rec.) (SH1237-F8)
330
CHF
CHF 330.00
In stock
AG-27B-6324-C05050 µgCHF 330.00
| Product Details | |
|---|---|
| Synonyms | Woronin Body Major Protein |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | SH1237-F8 |
| Isotype | Human IgG1 |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Synthetic biotinylated Hex-1 (Neurospora crassa) peptide. |
| Application |
ELISA |
| Crossreactivity | Neurospora crassa |
| Specificity |
Recognizes Hex1 from Neurospora crassa. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from animal component-free supernatant. |
| Concentration | 1 mg/ml |
| Formulation | Liquid. In PBS. |
| Isotype Negative Control | |
| Other Product Data |
This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Accession Number | P87252 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 1 week when stored at +4°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Neurospora crassa has been an excellent model organism for eukaryotic genetics and biochemistry and one of the workhorses for fungal cell biology research. The rapid (mm~4 hr/1) filamentous growth habit of N. crassa is the result of a strongly polarized mechanism culminating in the biogenesis of the tubular cell wall. Woronin bodies (WBs)peroxisome-related are membrane-bound organelles of filamentous ascomycetes that mediate hyphal compartmentalization by plugging septal pores upon hyphal damage. Their major component is the peroxisomal protein HEX-1, which has also been implicated in additional cellular processes in fungi.
Product References
- Linear Discriminant Analysis Identifies Mitochondrially Localized Proteins in Neurospora crassa: L. Wirsing, et al.; J. Proteome Res. 14, 3900 (2015)