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AdipoGen Life Sciences
anti-HA-Tag, mAb (rec.) (AB158-1-D11) (Fc Rabbit)
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| Product Details | |
|---|---|
| Synonyms | Hemagglutinin |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | AB158-1-D11 |
| Isotype | Rabbit IgG |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | HA peptide corresponding to HA-tag (Human influenza hemagglutinin), aa 98-106, YPYDVPDYA. |
| Application |
Western Blot: 0.2-5 µg/mL ELISA: 1-12 µg/mL as coating antibody and 0.5-5 µg/mL as detection antibody Immunofluorescence (IF): 0.1 - 10 µg/mL Optimal working dilution should be determined by the investigator. The actual dilution used must be determined experimentally for each assay. Dilution factors are presented in the form of a range because the optimal dilution varies by several factors. |
| Specificity | Recognizes the HA peptide corresponding to HA-tag (Human influenza hemagglutinin) (aa 98-106). |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from HEK293 cell culture supernatant. |
| Concentration | 1 mg/mL |
| Formulation | Liquid. In PBS. Contains 0,02% ProClin300. |
| Isotype Negative Control | |
| Other Product Data | This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Accession Number | A0A1B2G3M1 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 1 week when stored at +4°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins. This antibody can be detected with anti-rabbit Fc secondary antibodies.