AdipoGen Life Sciences

anti-Tetrahydrocannabinol (THC), mAb (rec.) (AB499-A06)

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Product Details
Synonyms THC; Δ9-Tetrahydrocannabinol; NSC 134454
Product Type Recombinant Antibody
Properties
Clone AB499-A06
Isotype Mouse IgG2a
Source/Host Produced without the use of animals. Purified from HEK 293 cell culture supernatant.
Immunogen/Antigen Tetrahydrocannabinol (THC) peptide.
Application ELISA: 1-12 µg/mL as coating antibody and 0.5-5 µg/mL as detection antibody
Optimal working dilution should be determined by the investigator. The actual dilution used must be determined experimentally for each assay. Dilution factors are presented in the form of a range because the optimal dilution varies by several factors.
Specificity Recognizes tetrahydrocannabinol (THC). No cross-reactivity to Cannabidiol (CBD).
Purity ≥95% (SDS-PAGE)
Purity Detail Protein A purified from HEK293 cell culture supernatant.
Concentration 1 mg/mL
Formulation Liquid. In PBS. Contains 0,02% ProClin300.
Isotype Negative Control

Mouse IgG2a Isotype Control

Other Product Data This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Declaration Manufactured by Abcalis
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Please handle under sterile conditions to avoid contamination.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Stable for at least 1 week when stored at +4°C.
Documents
Product Specification Sheet
Datasheet Download PDF
Description
Tetrahydrocannabinol (THC) is a is a cannabinoid found in cannabis. It is a psychoactive constituent of Cannabis L. THC usually refers to the delta-9-THC isomer, although 113 cannabinoids with the same chemical formula are described. This antibody detects Tetrahydrocannabinol (THC) but does not cross react with Cannabidiol (CBD). This antibody has no psychoactive activity and can be detected with anti-mouse Fc secondary antibodies.
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