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AdipoGen Life Sciences
anti-IgG4 (human), mAb (rec.) (AB131-2-C08)
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| Product Details | |
|---|---|
| Synonyms | Human Immunoglobulin G4; Immunoglobulin Heavy Constant Gamma 4 |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | AB131-2-C08 |
| Isotype | Mouse IgG2a |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Human IgG4. |
| Application |
ELISA: 1-12 µg/mL as coating antibody and 0.5-5 µg/mL as detection antibody Optimal working dilution should be determined by the investigator. The actual dilution used must be determined experimentally for each assay. Dilution factors are presented in the form of a range because the optimal dilution varies by several factors. |
| Specificity | Recognizes human IgG4. No cross-reactivity with the other human IgG subtypes IgG1, IgG2 and IgG3. Cross-reacts with goat IgG. Other species were not tested. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from HEK293 cell culture supernatant. |
| Concentration | 1 mg/mL |
| Formulation | Liquid. In PBS. Contains 0,02% ProClin300. |
| Isotype Negative Control | |
| Other Product Data | This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Accession Number | P01861 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 1 week when stored at +4°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
This antibody reacts with human IgG4 as determined by ELISA techniques. This antibody does not cross react with other human IgG subtypes like IgG1, IgG2 and IgG3. Anti-human monoclonal antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulin 4. This antibody works excellent in detection of human IgG4 and is useful in the detection, sorting or purification of its specified target. This antibody can be detected with anti-mouse Fc secondary antibodies.