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AdipoGen Life Sciences
anti-IgG (rabbit), mAb (rec.) Multiclonal
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| Product Details | |
|---|---|
| Synonyms | Rabbit Immunoglobulin G |
| Product Type | Recombinant Antibody |
| Properties | |
| Isotype | Mouse IgG2a |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Rabbit IgG. |
| Application |
Western Blot: 0.2-5 µg/mL ELISA: 1-12 µg/mL as coating antibody and 0.5-5 µg/mL as detection antibody Immunofluorescence (IF): 0.1 - 10 µg/mL Optimal working dilution should be determined by the investigator. The actual dilution used must be determined experimentally for each assay. Dilution factors are presented in the form of a range because the optimal dilution varies by several factors. |
| Specificity | Recognizes rabbit IgG. No cross-reactivity to mouse IgG, human IgG, goat IgG. Other species were not tested. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from HEK293 cell culture supernatant. |
| Concentration | 1 mg/mL |
| Formulation | Liquid. In PBS. Contains 0,02% ProClin300. |
| Isotype Negative Control | |
| Other Product Data | Multiclonal antibodies are developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Accession Number | P01870 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 1 week when stored at +4°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. These multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all subclasses of rabbit IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products.