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AdipoGen Life Sciences
anti-Calprotectin (human), mAb (rec.) (MS130i-IIIC3)
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| Product Details | |
|---|---|
| Synonyms | S100A8/S100A9; Calgranulin |
| Product Type | Recombinant Antibody |
| Properties | |
| Clone | AB467-1-A05 |
| Isotype | Mouse IgG2a |
| Source/Host | Produced without the use of animals. Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Recombinant human calprotectin. |
| Application |
ELISA: 1-12 µg/mL as coating antibody and 0.5-5 µg/mL as detection antibody Optimal working dilution should be determined by the investigator. The actual dilution used must be determined experimentally for each assay. Dilution factors are presented in the form of a range because the optimal dilution varies by several factors. |
| Specificity | Recognizes human calprotectin. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified from HEK293 cell culture supernatant. |
| Concentration | 1 mg/mL |
| Formulation | Liquid. In PBS. Contains 0,02% ProClin300. |
| Isotype Negative Control | |
| Other Product Data | This is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. |
| Accession Number | P05109-P06702 |
| Declaration | Manufactured by Abcalis |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Please handle under sterile conditions to avoid contamination. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Stable for at least 1 week when stored at +4°C. |
| Documents | |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Calprotectin is a functional heterocomplex of small EF hand calcium binding proteins S100A8 and S100A9. The heterocomplex is expressed in various cells and tissues and induced upon inflammation. The complex is released by neutrophilic granulocytes in inflammatory disorders such as inflammatory bowel disease and rheumatoid arthritis. Released calprotectin serves as a leukocyte chemoattractant and modulates inflammation via damage receptors. Via its binding to the receptor of advanced glycation end-products (RAGE), calprotectin is implicated in chronic inflammation and was also linked to cancer formation. This antibody can be detected with anti-mouse Fc secondary antibodies.