Streptavidin (HRP)

Specifications / Handling

More Information
Product Details
Product Type	Protein
Properties
Source/Host	E. coli
Sequence	Recombinant streptavidin from Streptomyces avidinii.
Crossreactivity	All
Application	ELISA, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Western Blotting
Label/Conjugates	Horseradish peroxidase
Specificity	Binds to any biotinylated reagents.
MW	60kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Concentration	1mg/ml after reconstitution.
Reconstitution	Reconstitute with 50μl endotoxin-free water.
Accession Number	P22629
Formulation	Lyophilized. Contains PBS.
Other Product Data	UniProt link P22629: Streptavidin
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Streptavidin is a non-glycosylated protein isolated from Streptomyces avidinii. It forms a homotetramer and strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). Streptavidin (HRP) is useful as a second step reagent for indirect enzymatic labeling, in conjunction with biotinylated primary antibodies. It is often used in ELISA, IHC or Western blotting applications together with relevant substrate systems.

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