Asc Antibody (AL177) Blocking Peptide
To use the blocking peptide, the following procedure is recommended:
Step 1: Mix 1μg/ml of the anti-Asc, pAb (AL177) (Prod. No. AG-25B-0006) and 10μg/ml of the blocking peptide together in the final desired volume (e.g. 5ml or 10ml for a western blotting).
Step 2: Incubate for 30min at room temperature with occasional mixing.
Step 3: Apply the antibody/peptide mixture (from step 1) to the membrane to perform the western blotting as usual.
|Formulation||Liquid. In sterile water.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
|Product Specification Sheet|
Blocking Peptide for anti-Asc, pAb (AL177). This vial contains 100µg peptide in 100µl sterile water. The Asc Antibody (AL177) Blocking Peptide can be used in conjunction with several variants of the anti-Asc pAb (AL177) (Prod. No. AG-25B-0006) to block protein-antibody complex formation.