anti-Asc, pAb (AL177) (preservative free)

CHF 490.00
In stock
AG-25B-0006PF-C100100 µgCHF 490.00
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Product Details
Synonyms Pycard; Target of Methylation-induced Silencing 1; TMS1; Caspase Recruitment Domain-containing Protein 5; Apoptosis-associated Speck-like Protein Containing CARD; CARD5
Product Type Polyclonal Antibody
Properties
Source/Host Rabbit
Immunogen/Antigen Synthetic peptide corresponding to aa at the N-terminal human Asc.
Label/Conjugates Preservative Free
Application

Immunocytochemistry: (1:200)
Immunohistochemistry: (1:500; paraffin sections)
Immunoprecipitation: (1:200)
Western Blot: (1:1000)
Functional Application: (see Protocol)

The antibody AL177 can be used to measure inflammasome activation in vivo, detecting Asc speck formation by immunofluorescence.

Crossreactivity Human
Mouse
Specificity

Recognizes human and mouse Asc.

Purity Detail Epitope-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS.
Isotype Negative Control

Rabbit IgG Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Asc promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. It is a component of the inflammasome, a protein complex which also includes NLRP3/NALP3, CARD8 and CASP1 and whose function is the activation of proinflammatory caspases.

Product References
  1. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini, et al.; Immunity 20, 319 (2004)
  2. P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1beta in Mouse Macrophage: P. Pelegrin, et al.; J. Immunol. 180, 7147 (2008)
  3. Inflammatory role of ASC in antigen-induced arthritis is independent of caspase-1, NALP-3, and IPAF: L. Kolly, et al.; J. Immunol. 183, 4003 (2009) (IF)
  4. Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012)
  5. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 674 (2013) (IF)
  6. Measuring inflammasome activation in response to bacterial infection: P. Broz & D.M. Monack; Methods Mol. Biol. 1040, 65 (2013)
  7. Promyelocytic leukemia protein interacts with the apoptosis-associated speck-like protein to limit inflammasome activation: J.K. Dowling, et al.; J. Biol. Chem. 289, 6429 (2014)
  8. Localization and functionality of the inflammasome in neutrophils: M. Bakele, et al.; J. Biol. Chem. 289, 5320 (2014)
  9. The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014) (IF)
  10. The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)
  11. Aging-associated TNF production primes inflammasome activation and Nlrp3-related metabolic disturbances: F. Bauernfeind, et al.; J. Immunol. 197, 2900 (2016)
  12. Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence: M. Beilharz, et al.; Methods Mol. Biol. 1417, 145 (2016)
  13. Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016)
  14. The inflammasome pathway in stable COPD and acute exacerbations: R. Faner, et al.; ERJ Open Res. 2, 00002-2016 (2016) (IF)
  15. A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly: F.I. Schmidt, et al.; J. Exp. Med. 213, 771 (2016) (IF)
  16. Microglia-derived ASC specks cross-seed amyloid-β in Alzheimer's disease: C. Venegas, et al.; Nature 552, 355 (2017) (IF)
  17. The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer: E. Brunetto, et al.; J. Immunother. Cancer 7, 45 (2019) (IF)
  18. NLRP3 inflammasome activation drives tau pathology: C. Ising, et al.; Nature 575, 669 (2019) (IF)
  19. Human NLRP1 is a sensor for double-stranded RNA: S. Bauernfried, et al.; Science 371, 482 (2021)
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