anti-Asc, pAb (AL177) (preservative free)

CHF 490.00
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AG-25B-0006PF-C100100 µgCHF 490.00
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Product Details
Synonyms Pycard; Target of Methylation-induced Silencing 1; TMS1; Caspase Recruitment Domain-containing Protein 5; Apoptosis-associated Speck-like Protein Containing CARD; CARD5
Product Type Polyclonal Antibody
Source/Host Rabbit
Immunogen/Antigen Synthetic peptide corresponding to aa at the N-terminal human Asc.
Label/Conjugates Preservative Free

Immunocytochemistry: (1:200)
Immunohistochemistry: (1:500; paraffin sections)
Immunoprecipitation: (1:200)
Western Blot: (1:1000)
Functional Application: (see Protocol)

The antibody AL177 can be used to measure inflammasome activation in vivo, detecting Asc speck formation by immunofluorescence.

Crossreactivity Human

Recognizes human and mouse Asc.

Purity Detail Epitope-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS.
Isotype Negative Control

Rabbit IgG Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Asc promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. It is a component of the inflammasome, a protein complex which also includes NLRP3/NALP3, CARD8 and CASP1 and whose function is the activation of proinflammatory caspases.

Product References
  1. NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder: L. Agostini, et al.; Immunity 20, 319 (2004)
  2. P2X7 Receptor Differentially Couples to Distinct Release Pathways for IL-1beta in Mouse Macrophage: P. Pelegrin, et al.; J. Immunol. 180, 7147 (2008)
  3. Inflammatory role of ASC in antigen-induced arthritis is independent of caspase-1, NALP-3, and IPAF: L. Kolly, et al.; J. Immunol. 183, 4003 (2009) (IF)
  4. Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012)
  5. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 674 (2013) (IF)
  6. Measuring inflammasome activation in response to bacterial infection: P. Broz & D.M. Monack; Methods Mol. Biol. 1040, 65 (2013)
  7. Promyelocytic leukemia protein interacts with the apoptosis-associated speck-like protein to limit inflammasome activation: J.K. Dowling, et al.; J. Biol. Chem. 289, 6429 (2014)
  8. Localization and functionality of the inflammasome in neutrophils: M. Bakele, et al.; J. Biol. Chem. 289, 5320 (2014)
  9. The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014) (IF)
  10. The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)
  11. Aging-associated TNF production primes inflammasome activation and Nlrp3-related metabolic disturbances: F. Bauernfeind, et al.; J. Immunol. 197, 2900 (2016)
  12. Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence: M. Beilharz, et al.; Methods Mol. Biol. 1417, 145 (2016)
  13. Cell-Free Assay for Inflammasome Activation: Y. Jamilloux & F. Martinon; Methods Mol. Biol. 1417, 207 (2016)
  14. The inflammasome pathway in stable COPD and acute exacerbations: R. Faner, et al.; ERJ Open Res. 2, 00002-2016 (2016) (IF)
  15. A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly: F.I. Schmidt, et al.; J. Exp. Med. 213, 771 (2016) (IF)
  16. Microglia-derived ASC specks cross-seed amyloid-β in Alzheimer's disease: C. Venegas, et al.; Nature 552, 355 (2017) (IF)
  17. The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer: E. Brunetto, et al.; J. Immunother. Cancer 7, 45 (2019) (IF)
  18. NLRP3 inflammasome activation drives tau pathology: C. Ising, et al.; Nature 575, 669 (2019) (IF)
  19. Human NLRP1 is a sensor for double-stranded RNA: S. Bauernfried, et al.; Science 371, 482 (2021)
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