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AdipoGen Life Sciences
Adiponectin (rat) (rec.)
395
CHF
CHF 395.00
In stock
AG-40A-0004Y-C05050 µgCHF 395.00
Figure 1: Effect of Adiponectin (rat) (rec.) (Prod. No. AG-40A-0004Y) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.
Method: C2C12 myocytes were treated with 25μg/ml for 15min after serum starvation for 24hr. 100μg per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.
1: 25μg/ml of Adiponectin (rat) (rec.).
2: 25μg/ml of a FLAG®-tagged control protein.
3: phosphate-buffered saline.
Method: C2C12 myocytes were treated with 25μg/ml for 15min after serum starvation for 24hr. 100μg per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.
1: 25μg/ml of Adiponectin (rat) (rec.).
2: 25μg/ml of a FLAG®-tagged control protein.
3: phosphate-buffered saline.
| Product Details | |
|---|---|
| Synonyms | ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Rat adiponectin (aa 16-244) is fused at the N-terminus to a FLAG®-tag. |
| Crossreactivity | Rat |
| Biological Activity |
Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. |
| MW | ~30kDa (SDS-PAGE); ~180kDa (Hexamer, by SEC) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 50μl endotoxin-free water. |
| Accession Number | Q8K3R4 |
| Formulation | Lyophilized. Contains 30mM Tris-Cl, pH 8.0 + 100mM NaCl. |
| Other Product Data |
UniProt link Q8K3R4: Adiponectin (rat) (precursor) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
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