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AdipoGen Life Sciences
DLL1 (human) (rec.)
190
CHF
CHF 190.00
In stock
AG-40A-0073-C01010 µgCHF 190.00
AG-40A-0073-C05050 µgCHF 380.00
Figure 1: Adipogenesis inhibition of MSCs.
MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1μM Dexamethasone, 0.5mM IBMX, 10μg/m lnsulin, 100μM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-α (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were first incubated with a solution of anti-FLAG® antibody (10μg/ml) in PBS for 30 mins at 37°C. Coated plates were blocked with growth medium for at least 30mins, incubated with solution of DLL1 (human) (rec.) (Prod. No. AG-40A-0073) (5μg/ml) or mCD137L-FLAG® (5μg/ml) in growth medium for 2 hours at 37°C. Plates were then used to differentiate MSCs.
MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1μM Dexamethasone, 0.5mM IBMX, 10μg/m lnsulin, 100μM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-α (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were first incubated with a solution of anti-FLAG® antibody (10μg/ml) in PBS for 30 mins at 37°C. Coated plates were blocked with growth medium for at least 30mins, incubated with solution of DLL1 (human) (rec.) (Prod. No. AG-40A-0073) (5μg/ml) or mCD137L-FLAG® (5μg/ml) in growth medium for 2 hours at 37°C. Plates were then used to differentiate MSCs.
| Product Details | |
|---|---|
| Synonyms | Delta-like Protein 1; Delta1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | Signal peptide and extracellular domain of human DLL1 (aa 1-545) are fused at the C-terminus to a FLAG®-tag. |
| Crossreactivity | Human |
| Biological Activity | Inhibits adipogenesis of mesenchymal stem cells. |
| MW | ~70kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | ~0.3mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS. |
| Other Product Data |
UniProt link O00548: DLL1 (human) [Precursor] FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Notch ligand delta-like protein 1 (DLL1) is essential for postnatal arteriogenesis and contributes to tumor progression. DLL1 is involved in differentiation and self-renewal of adipocyte stem cells. Blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK-cell precursor.
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