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AdipoGen Life Sciences
DLL1 (human):Fc (human) (rec.)
305
CHF
CHF 305.00
In stock
AG-40A-0116Y-C01010 µgCHF 305.00
AG-40A-0116Y-C05050 µgCHF 835.00
Figure 1: Adipogenesis inhibition of 3T3L1 cells.
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1μM dexamethasone, 0.5mM IBMX, 10μg/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml hDLL1-Fc (Prod. No. AG-40A-0116Y) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1μM dexamethasone, 0.5mM IBMX, 10μg/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml hDLL1-Fc (Prod. No. AG-40A-0116Y) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Figure 2: Adipogenesis inhibition. Macroscopic pictures.
Figure 3: Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
| Product Details | |
|---|---|
| Synonyms | Delta-like Protein 1; Delta1 |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
Human DLL1 extracellular domain (aa 18-545) is fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Human |
| Specificity |
Interacts with human Notch1 (as confirmed by flow cytometry). |
| Biological Activity |
Inhibits adipogenesis of 3T3L-1 cells. Induces the Notch target gene HES-1 when coated on a plate at 1µg/ml. |
| MW | ~100kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 50µg size: 1 mg/ml |
| Reconstitution |
10µg size: Reconstitute with 100µl sterile water. 50µg size: Reconstitute with 50µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control | |
| Other Product Data |
Uniprot link O00548: DLL1 (human) [Precursor] |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
DLL1 (Delta-like protein; Delta1) is essential for postnatal arteriogenesis and contributes to tumor progression. DLL1 is involved in differentiation and self-renewal of adipocyte stem cells. Blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK-cell precursor.
Product References
- Jagged2 acts as a Delta-like Notch ligand during early hematopoietic cell fate decisions: I. Van de Walle, et al.; Blood 117, 4449 (2011)
- Differential regulation of osteoclastogenesis by Notch2/Delta-like 1 and Notch1/Jagged1 axes: C. Sekine, et al.; Arthr. Res. Ther. 14, R45 (2012)
- Canonical Notch signalling is inactive in urothelial carcinoma: A. Greife, et al.; BMC Cancer 14, 628 (2014)
- Notch Signaling Promotes Osteoclast Maturation and Resorptive Activity: J.W. Ashley, et al,; J. Cell Biochem. 116, 2598 (2015)
- Notch ligands regulate the muscle stem-like state ex vivo but are not sufficient for retaining regenerative capacity: H. Sakai, et al.; PLoS ONE 12, e0177516 (2017)
- Notch regulates innate lymphoid cell plasticity during human NK cell development: A.P. Nalin, et al.; J. Immunol. 205, 2679 (2020)
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