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AdipoGen Life Sciences
DLL4 (human):Fc (human) (rec.) (highly active mutant)
350
CHF
CHF 350.00
In stock
AG-40B-0176-C01010 µgCHF 350.00
AG-40B-0176-C100100 µgCHF 1'080.00
Figure 1: DLL4 (human):Fc (human) (rec.) (highly active mutant) (AG-40B-0176) binds to mNotch1 with higher affinity than WT DLL4:Fc. Method: DLL4 (human):Fc (human) (highly active mutant) (AG-40B-0176) or DLL4 (human):Fc (human) WT (AG-40A-0077Y) was coated on an ELISA plate at 1μg/ml. After blocking and washing steps, indicated concentrations of Notch1 (mouse):Fc were added. Following incubation for 1 hour at RT, the binding was detected using an anti-Notch1 (mouse) primary antibody (AG-20B-0051), following with an anti-IgG (rat) (HRP) secondary antibody.
| Product Details | |
|---|---|
| Synonyms | Delta-like Protein 4; Delta4 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Extracellular domain of human DLL4 (aa 27-529 plus mutations) are fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity |
Human Mouse |
| Specificity |
Interacts with human and mouse Notch1 with >20 fold increase in affinity relative to WT DLL4 (as confirmed by ELISA). |
| MW | ~100kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1 mg/ml |
| Reconstitution | Reconstitute with 100µl sterile endotoxin-free water. |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control | |
| Other Product Data |
UniProt link Q9NR61: DLL4 (human) [Precursor] |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, promoting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.
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