DLL4 (human):Fc (human) (rec.) (highly active mutant)
|Synonyms||Delta-like Protein 4; Delta4|
|Source/Host||HEK 293 cells|
|Sequence||Extracellular domain of human DLL4 (aa 27-529 plus mutations) are fused at the C-terminus to the Fc portion of human IgG1.|
|Specificity||Interacts with human Notch1 with >20 fold increase in affinity relative to WT DLL4 (as confirmed by ELISA).|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
10µg size: 0.1mg/ml after reconstitution.
100µg size: 1mg/ml after reconstitution.
|Reconstitution||Reconstitute with 100µl sterile endotoxin-free water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||UniProt link Q9NR61: DLL4 (human) [Precursor]|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, promoting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.