DLL4 (human):Fc (human) (rec.) (highly active mutant)
AG-40B-0176-C01010 µgCHF 370.00
AG-40B-0176-C100100 µgCHF 1'200.00
DLL4 (human):Fc (human) (rec.) (highly active mutant) (AG-40B-0176) binds to mNotch1 with higher affinity than WT DLL4:Fc. Method: Notch1 (mouse):Fc was coated on an ELISA plate at 1μg/ml. After blocking and washing steps, indicated concentrations of DLL4 (human):Fc (human) (highly active mutant) (AG-40B-0176) and DLL4 (human):Fc (human) WT (AG-40A-0077Y) were added. Following incubation for 1 hour at RT, the binding was detected using an anti-Notch1 (mouse) primary antibody (AG-20B-0051), following with an anti-IgG (rat) (HRP) secondary antibody.
|Synonyms||Delta-like Protein 4; Delta4|
|Source/Host||HEK 293 cells|
|Sequence||Extracellular domain of human DLL4 (aa 27-529 plus mutations) are fused at the C-terminus to the Fc portion of human IgG1.|
|Specificity||Interacts with human Notch1 with >20 fold increase in affinity relative to WT DLL4 (as confirmed by ELISA).|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
10µg size: 0.1mg/ml after reconstitution.
100µg size: 1mg/ml after reconstitution.
|Reconstitution||Reconstitute with 100µl sterile endotoxin-free water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||UniProt link Q9NR61: DLL4 (human) [Precursor]|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
|Use/Stability||Stable for at least 6 months after receipt when stored at -20°C.|
|Product Specification Sheet|
The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, promoting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.