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AdipoGen Life Sciences
DLL4 (mouse):Fc (human) (rec.)
As low as
130
CHF
CHF 130.00
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AG-40A-0145-C01010 µgCHF 130.00
AG-40A-0145-C05050 µgCHF 290.00
Figure 1: Adipogenesis inhibition of 3T3L1 cells.
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1μM Dexamethasone, 0.5mM IBMX, 10μg/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFα and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1μM Dexamethasone, 0.5mM IBMX, 10μg/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFα and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
| Product Details | |
|---|---|
| Synonyms | Delta-like Protein 4; Delta4 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Signal peptide and extracellular domain of mouse DLL4 (aa 1-532) is fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Mouse |
| Biological Activity |
Inhibits adipogenesis of 3T3L-1 cells. |
| MW | ~95kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Formulation | Liquid. 0.2μm-filtered solution in PBS. |
| Protein Negative Control | |
| Other Product Data |
UniProt link Q9JI71: DLL4 (mouse) [Precursor] |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, pro-moting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.
Product References
- Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression: M. Reis, et al.; J. Exp. Med. 209, 1611 (2012)
- Flt-1 (vascular endothelial growth factor receptor-1) is essential for the vascular endothelial growth factor-Notch feedback loop during angiogenesis: J.C. Chappell, et al.; Arterioscler. Thromb. Vasc. Biol. 33, 1952 (2013)
- Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFβ, and extracellular matrix: K.B. Kaylan, et al.; Scientific Rep. 6, 23490 (2016)
- Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling: K.B. Kaylan, et al.; Elife 7, e38536 (2018)
- Reciprocal communication between astrocytes and endothelial cells is required for astrocytic glutamate transporter 1 (GLT-1) expression: Z. Martinez-Lozada & M.B. Robinson; Neurochem. 139, 104787 (2020)
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