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Figure 1: Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1μM Dexamethasone, 0.5mM IBMX, 10μg/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFα and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.
Use/Stability
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, pro-moting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.
Product References
Endothelial Wnt/β-catenin signaling inhibits glioma angiogenesis and normalizes tumor blood vessels by inducing PDGF-B expression: M. Reis, et al.; J. Exp. Med. 209, 1611 (2012)
Flt-1 (vascular endothelial growth factor receptor-1) is essential for the vascular endothelial growth factor-Notch feedback loop during angiogenesis: J.C. Chappell, et al.; Arterioscler. Thromb. Vasc. Biol. 33, 1952 (2013)
Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFβ, and extracellular matrix: K.B. Kaylan, et al.; Scientific Rep. 6, 23490 (2016)
Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling: K.B. Kaylan, et al.; Elife 7, e38536 (2018)
Reciprocal communication between astrocytes and endothelial cells is required for astrocytic glutamate transporter 1 (GLT-1) expression: Z. Martinez-Lozada & M.B. Robinson; Neurochem. 139, 104787 (2020)
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