DLL4 (human):Fc (human) (rec.)

CHF 295.00
In stock
AG-40A-0077Y-C01010 µgCHF 295.00
AG-40A-0077Y-C05050 µgCHF 520.00
Replaces AG-40A-0077
More Information
Product Details
Synonyms Delta-like Protein 4; Delta4
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence Signal peptide and extracellular domain of human DLL4 (aa 1-529) are fused at the C-terminus to the Fc portion of human IgG1.
Crossreactivity Human
Specificity Interacts with human Notch1 (as confirmed by flow cytometry).
Biological Activity Inhibits adipogenesis of 3T3L-1 cells and mesenchymal stem cells (MSCs). Induces the Notch target gene HES-1 when coated on a plate at 1µg/ml.
MW ~80kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration 10µg size: 0.1mg/ml after reconstitution.
50µg size: 1mg/ml after reconstitution.
Reconstitution 10µg size: Reconstitute with 100µl sterile water.
50µg size: Reconstitute with 50µl sterile water.
Formulation Lyophilized. Contains PBS + 0.5 % Trehalose.
Other Product Data UniProt link Q9NR61: DLL4 (human) [Precursor]
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

The Notch ligand delta-like protein 4 (DLL4) is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch1 and Notch4. It is induced by VEGF as a negative feedback regulator and acts to prevent overexuberant angiogenic sprouting, promoting the timely formation of a well differentiated vascular network. DLL4-Notch1 signaling regulates the formation of appropriate numbers of tip cells to control vessel sprouting and branching in the mouse retina.

Product References
  1. Jagged2 acts as a Delta-like Notch ligand during early hematopoietic cell fate decisions: I. Van de Walle, et al.; Blood 117, 4449 (2011)
  2. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6: K.P. Mouillesseaux, et al.; Nat. Commun. 7, 13247 (2016)
  3. Notch ligands regulate the muscle stem-like state ex vivo but are not sufficient for retaining regenerative capacity: H. Sakai, et al.; PLoS ONE 12, e0177516 (2017)
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