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AdipoGen Life Sciences
FGF-23 (human) (rec.) (His)
230
CHF
CHF 230.00
In stock
AG-40A-0114Y-C01010 µgCHF 230.00
Figure 1: ERK and FRS2α phosphorylation induced by FGF-23 in Klotho expressing cells.
Klotho expressing HEK 293EBNA cells were serum starved for 16hr and then stimulated with hFGF-23-His (Prod. No. AG-40A-0114Y), mCD137-Fc (Fc control), and FGF-b (100ng/ml) (positive control) for 10 min, respectively. Antibodies against pFRS2α, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: Mock (non-treated) | Lane 2: Mock + hFGF23-Fc 1μg/ml
Lane 3: Mock + hFGF23-His 1μg/ml | Lane 4: Klotho (non-treated)
Lane 5: Klotho + 100ng/ml FGF-b | Lane 6: Klotho + hFGF23-His 0.3μg/ml
Lane 7: Klotho + hFGF23-His 1μg/ml | Lane 8: Klotho + hFGF23-His 4μg/ml
Klotho expressing HEK 293EBNA cells were serum starved for 16hr and then stimulated with hFGF-23-His (Prod. No. AG-40A-0114Y), mCD137-Fc (Fc control), and FGF-b (100ng/ml) (positive control) for 10 min, respectively. Antibodies against pFRS2α, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: Mock (non-treated) | Lane 2: Mock + hFGF23-Fc 1μg/ml
Lane 3: Mock + hFGF23-His 1μg/ml | Lane 4: Klotho (non-treated)
Lane 5: Klotho + 100ng/ml FGF-b | Lane 6: Klotho + hFGF23-His 0.3μg/ml
Lane 7: Klotho + hFGF23-His 1μg/ml | Lane 8: Klotho + hFGF23-His 4μg/ml
Figure 2: ERK phosphorylation and FRS2α phosphorylation in Klotho expressing cells induced by FGF23-His (Prod. No. AG-40A-0114Y) and FGF23-His (R179Q mutant) (Prod. No. AG-40A-0126Y).
Klotho expressing HEK293EBNA cells were serum starved for 16h. They were then stimulated with recombinant hFGF23-His, hFGF23-His (R179Q) and FGF-b for 10min respectively. FGF-b (100ng/ml) was used as positive control. The cell lysate was subjected to do immunoblotting with antibodies against pFRS2α, pERK1/2 and total ERK1/2.
Lane 1: Mock (non-treated) | Lane 2: Klotho + hFGF23-His (wt) 0.1μg/ml
Lane 3: Klotho + hFGF23-His (wt) 0.3μg/ml | Lane 4: Klotho + hFGF23-His (wt) 1μg/ml
Lane 5: Klotho + hFGF23-His (R179Q) 0.1μg/ml | Lane 6: Klotho + hFGF23-His (R179Q) 0.3μg/ml
Lane 7: Klotho + hFGF23-His (R179Q) 1μg/ml | Lane 8: Klotho + FGF-b 100ng/ml
Klotho expressing HEK293EBNA cells were serum starved for 16h. They were then stimulated with recombinant hFGF23-His, hFGF23-His (R179Q) and FGF-b for 10min respectively. FGF-b (100ng/ml) was used as positive control. The cell lysate was subjected to do immunoblotting with antibodies against pFRS2α, pERK1/2 and total ERK1/2.
Lane 1: Mock (non-treated) | Lane 2: Klotho + hFGF23-His (wt) 0.1μg/ml
Lane 3: Klotho + hFGF23-His (wt) 0.3μg/ml | Lane 4: Klotho + hFGF23-His (wt) 1μg/ml
Lane 5: Klotho + hFGF23-His (R179Q) 0.1μg/ml | Lane 6: Klotho + hFGF23-His (R179Q) 0.3μg/ml
Lane 7: Klotho + hFGF23-His (R179Q) 1μg/ml | Lane 8: Klotho + FGF-b 100ng/ml
| Product Details | |
|---|---|
| Synonyms | Fibroblast Growth Factor 23; Tumor-derived Hypophosphatemia-inducing Factor |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | Signal peptide and human FGF-23 (aa 1-251) are fused at the C-terminus to a His-tag. |
| Crossreactivity | Human |
| Biological Activity | Activates ERK and FRS2α phosphorylation in Klotho expressing cells. |
| MW | ~30kDa (SDS-PAGE, full-length). FGF23 is cleaved between Arg179 and Ser180 (two bands of ~14kDa and ~16kDa). |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data | UniProt link Q9GZV9: FGF-23 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
FGF-23 (Fibroblast growth factor 23) is a regulator of phosphate homeostasis. It upregulates EGR1 expression in the presence of KLBy. Acts directly on the parathyroid to decrease PTH secretion. Regulates the vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF-23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR) and of hyperphosphatemic familial tumoral calcinosis (HFTC).
Product References
- Maternally-derived antibody to fibroblast growth factor-23 reduced dietary phosphate requirements in growing chicks: E.A. Bobeck, et al.; BBRC 420, 666 (2012)
- Antibody to fibroblast growth factor 23-peptide reduces excreta phosphorus of laying hens: Z. Ren, et al.; Poultry Science 96, 127 (2017)
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