DLL1 (mouse):Fc (human) (rec.)
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1μM dexamethasone, 0.5mM IBMX, 10μg/m insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml mDLL1-Fc (Prod. No. AG-40A-0148Y) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin and stained with 0.5% filtered Oil Red O in propylene glycol.
A mouse preadipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Lane 1: mDLL1 (Fc protein), 0 min
Lane 2: mDLL1 (Fc protein), 10min
Lane 3: mDLL1 (Fc protein), 30min
Lane 4: mDLL1 (Fc protein), 1hr
Lane 5: mDLL1 (Fc protein), 2hr
Lane 6: mDLL1 (Fc protein), 4hr
Lane 7: mDLL1 (Fc protein), 8hr
Lane 8: mDLL1 (Fc protein), 24hr
|Synonyms||Delta-like Protein 1; Delta 1|
|Source/Host||HEK 293 cells|
|Sequence||Extracellular domain of mouse DLL1 (aa 18-545) is fused at the C-terminus to the Fc portion of human IgG1.|
|Specificity||Interacts with Notch receptors.|
|Biological Activity||Inhibits adipogenesis of 3T3L-1 cells. Induces Hes-1 in 3T3L-1 cells.|
|Endotoxin Content||<0.1EU/μg purified protein (LAL test; Lonza).|
for 10µg size: 0.1mg/ml
for 50µg size: 1 mg/ml
10µg size: Reconstitute with 100µl sterile water.
50µg size: Reconstitute with 50µl sterile water.
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||Uniprot link Q61483: DLL1 (mouse)|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
The Notch ligand delta-like protein 1 (DLL1) is essential for postnatal arteriogenesis and contributes to tumor progression. DLL1 is involved in differentiation and self-renewal of adipocyte stem cells. Blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK cell precursor.
- Flt-1 (Vascular Endothelial Growth Factor Receptor-1) Is Essential for the Vascular Endothelial Growth Factor-Notch Feedback Loop During Angiogenesis: J.C. Chappell, et al.; Arterioscler. Thromb. Vasc. Biol. 33, 1952 (2013)
- Mimicking The Stem Cell Niche In Diseased Muscle With A Notch Activator-conjugated 3d-construct: X. Mu, et al.; ORS Annual Meeting Transactions Presentation (2014)
- Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors: M. Weiss-Gayet, et al.; PLoS One 11, e0153860 (2016)