Progranulin (human) (rec.) (untagged)

CHF 195.00
In stock
AG-40A-0188Y-C01010 µgCHF 195.00
AG-40A-0188Y-C05050 µgCHF 430.00
Replaces AG-40A-0188
More Information
Product Details
Synonyms Proepithelin; PEPI; PC Cell-derived Growth Factor
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

Signal peptide and human progranulin (aa 1-593) is untagged. Reflects the native sequence with no additional aa.

Crossreactivity Human
Biological Activity

Binds to human sortilin. Activates phospho-ERK1/2 in neuronal mouse P19 cells. Regulates food intake and body weight (see reference 1).

MW ~90kDa (glycosylated protein by SDS-PAGE)
Purity ≥98% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 50µg size: 1 mg/ml
Reconstitution 10µg size: Reconstitute with 100µl sterile water.
50µg size: Reconstitute with 50µl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data

Uniprot link P28799: Granulins (human) [Precursor]

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Progranulin (PGRN) is a widely expressed pluripotent growth factor which plays a role in processes such as development, wound repair and inflammation by activating signaling cascades that control cell cycle progression and cell motility. Its function in the central nervous system is of interest, as mutations in the PGRN gene were found in cases of frontotemporal degeneration (FTLD). In addition, PGRN has also been linked to tumorigenesis. Progranulin is a biomarker for FTLD, other types of Alzheimer‘s Disease (AD) and potentially for MCI (Mild Cognitive Impairment). Additionally, PGRN is described as a new ligand of TNF receptors and a potential therapeutic against inflammatory disease like arthritis.  EphA2, a member of the large family of Ephrin receptor tyrosine kinases, is also a functional signaling receptor for progranulin as reported recently.

Product References
  1. Involvement of Progranulin in Hypothalamic Glucose Sensing and Feeding Regulation: H.K. Kim, et al.; Endocrinology 152, 4672 (2011)
  2. Progranulin directly binds to the CRD2 and CRD3 of TNFR extracellular domains: J. Jian, et al.; FEBS Lett. 587, 3428 (2013)
  3. Progranulin and a five transmembrane domain-containing receptor-like gene are the key components in receptor activator of nuclear factor B (RANK)-dependent formation of multinucleated osteoclasts: J. Oh, et al.; J. Biol. Chem. 290, 2042 (2015)
  4. New discovery rarely runs smooth: an update on progranulin/TNFR interactions: B.C. Wang, et al.; Protein Cell 6, 792 (2015)
  5. Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes: Y. Tanaka, et al.; Hum. Mol. Genet. 26, 969 (2017)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.