AdipoGen Life Sciences

CD40L (human) (multimeric) (rec.) (Animal Free)

CHF 390.00
In stock
AG-40B-0010AF-C01010 µgCHF 390.00
AG-40B-0010AF-C100100 µgCHF 1'800.00
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Product Details
Synonyms MultimericCD40L™ (Animal Free); ACRP30headless:CD40L; ACRP30headless:CD154; ADIPOQ-CD40L; ACRP30headless:TNFSF5
Product Type Protein
Source/Host CHO cells. Produced using animal component-free medium.

Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Crossreactivity Human

Can be used for B cell expansion or during TIL expansion to increase the activation of T cells.


Binds to human CD40.

Biological Activity

Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml). B cell expansion online protocol available.

MW ~35-40kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test, Lonza).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 100µg size: 1mg/ml
Reconstitution Reconstitute with 100μl endotoxin-free water.
Accession Number P29965
Formulation Lyophilized. Contains PBS
Other Product Data

UniProt link P29965: CD40L (human)
UniProt link Q60994: ACRP30 (mouse) (precursor)

FLAG is a registered trademark of Sigma-Aldrich Co.

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
MSDS Inquire
Product Specification Sheet
Datasheet Download PDF

CD40 ligand (CD40L) expressed by activated T cells engages CD40 expressed by B cells and APCs. CD40L can provide signals to B cells that induce proliferation, immunoglobulin class switching and antibody secretion. CD40L has been used recently in Immunotherapy to activate tumor-infiltrated lymphocytes (TIL).

MultimericCD40L™ is a high-activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L™ has been shown to be a potent tool for B cell expansion. It also has big potential as a growth factor for tumor-infiltrating lymphocytes (TILs), which have been shown to be important for T cell therapy.

The CD40L (human) (multimeric) (rec.) (Animal Free) has been produced in CHO in suspension using animal-component free medium and certified CHO cells.

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