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AdipoGen Life Sciences
B-Xpander™ CD40L (human) (rec.) (Animal Free)
390
CHF
CHF 390.00
In stock
AG-40B-0010AF-C01010 µgCHF 390.00
AG-40B-0010AF-C100100 µgCHF 1'800.00
Figure 1: Measurement of B cell proliferation. METHOD: Activity of B-Xpander™ and 2 different research grades of Multimeric hCD40L is measured by proliferation of human primary CD19+ B cells. Cells (5 x 10^4/well) are grown in RPMI medium, glutamine, 10% FBS, IL-4 (human) (10ng/ml), IL-21 (human) (10ng/ml) and different concentrations of B-Xpander™ CD40L (human) (rec.) (Animal-Free) (#AG-40B-0010AF), CD40L (human) (multimeric) (rec.) (CSG; Certified Serum Grade) (#AG-40B-0010CSG), CD40L (human) (multimeric) (rec.) (#AG-40B-0010), or a control protein. Cell proliferation is quantified after 4 days using PMS/MTS (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Promega). Cells expanded by 10-fold after 4 days to reach 5 x 10^5/well.
Figure 2: B-Xpander™ (Prod. No. AG-40B-0010AF) induces B cell activation. METHOD: PBL cells were incubated in 96-well plates (2x10^5 cells/well in 100μl RPMI supplemented with 10% FCS) for 24 hours at 37°C with the indicated concentration of B-Xpander™ CD40L (human) (rec.) (Animal Free) (#AG-40B-0010AF), CD40L (human) (multimeric) (rec.) (#AG-40B-0010) or a trimeric human CD40L as a control. Cells were washed with PBS and stained with 2μl each CD86-PE and CD19-FITC in 50μl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) for 20 min. at 4°C in the dark. After two washes in FACS buffer, samples were then analyzed by flow cytometry.
Figure 3: Structure of the B-Xpander™ or Multimeric human CD40L.
| Product Details | |
|---|---|
| Synonyms | B-Xpander™ MultimericCD40L™ (Animal Free); ACRP30headless:CD40L; ACRP30headless:CD154; ADIPOQ-CD40L; ACRP30headless:TNFSF5 |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells. Produced using animal component-free medium. |
| Sequence |
Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
| Crossreactivity | Human |
| Application |
Can be used for B cell expansion or during TIL expansion to increase the activation of T cells. |
| Specificity |
Binds to human CD40. |
| Biological Activity |
Induces B cell activation and B cell expansion (concentrations depend on the protocol, but we recommend to start at 50-100 ng/ml). |
| MW | ~35-40kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test, Lonza). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml |
| Reconstitution | Reconstitute with 100μl endotoxin-free water. |
| Accession Number | P29965 |
| Formulation | Lyophilized. Contains PBS |
| Other Product Data |
UniProt link P29965: CD40L (human) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
B-Xpander™ is a potent enhanced Multimeric human CD40L cytokine, for B cell activation, B cell expansion (proliferation) and cell therapy applications. In-house production using a proprietary protocol and a characterized and certified CHO cell line allows the supply of the most active Multimeric human CD40L proteins in GMP-like format.
Interest in B cells has recently increased in immunotherapies using Engineered B cells or Tumor-Infiltrating Lymphocytes (TILs). However, current methods for B cell expansion are inefficient, time consuming and can lead to insufficient B cell numbers and persistent T cell exhaustion. Our B-Xpander™ protein improves current protocols for B cell stimulation by mimicking the natural interaction between CD40L and CD40 molecules on B cells.
B-Xpander™ Quality Features:
• Production in characterized and certified CHO cell line (for GMP-grade manufacturing)*
• High Bioactivity tested by ELISA/Cell-based Assays
• Verified Purity & Homogeneity by SEC
• Low Endotoxin Level
• Batch-to-Batch Consistency
• Animal-component free Production
• No cross-linking Reagents necessary
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