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AdipoGen Life Sciences
CD40L (human) (multimeric) (rec.) (Animal Free)
390
CHF
CHF 390.00
In stock
AG-40B-0010AF-C01010 µgCHF 390.00
AG-40B-0010AF-C100100 µgCHF 1'800.00
| Product Details | |
|---|---|
| Synonyms | MultimericCD40L™ (Animal Free); ACRP30headless:CD40L; ACRP30headless:CD154; ADIPOQ-CD40L; ACRP30headless:TNFSF5 |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells. Produced using animal component-free medium. |
| Sequence |
Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
| Crossreactivity | Human |
| Application |
Can be used for B cell expansion or during TIL expansion to increase the activation of T cells. |
| Specificity |
Binds to human CD40. |
| Biological Activity |
Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml). B cell expansion online protocol available. |
| MW | ~35-40kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test, Lonza). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml |
| Reconstitution | Reconstitute with 100μl endotoxin-free water. |
| Accession Number | P29965 |
| Formulation | Lyophilized. Contains PBS |
| Other Product Data |
UniProt link P29965: CD40L (human) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
CD40 ligand (CD40L) expressed by activated T cells engages CD40 expressed by B cells and APCs. CD40L can provide signals to B cells that induce proliferation, immunoglobulin class switching and antibody secretion. CD40L has been used recently in Immunotherapy to activate tumor-infiltrated lymphocytes (TIL).
MultimericCD40L™ is a high-activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L™ has been shown to be a potent tool for B cell expansion. It also has big potential as a growth factor for tumor-infiltrating lymphocytes (TILs), which have been shown to be important for T cell therapy.
The CD40L (human) (multimeric) (rec.) (Animal Free) has been produced in CHO in suspension using animal-component free medium and certified CHO cells.
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