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AdipoGen Life Sciences
Flagellin (high purity)
210
CHF
CHF 210.00
In stock
AG-40B-0025-C01010 µgCHF 210.00
AG-40B-0025-30103 x 10 µgCHF 420.00
Figure 1: Flagellin (AG-40B-0025) activates TLR5 in vivo.
Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10µg of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).
Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10µg of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).
| Product Details | |
|---|---|
| Synonyms | FliC |
| Product Type | Protein |
| Properties | |
| Source/Host | Salmonella typhimurium strain 14028 |
| Crossreactivity |
Human Mouse |
| Specificity |
Binds to human and mouse TLR5 (Toll-like receptor 5). |
| Biological Activity |
Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, β-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse. |
| MW | ~54kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.001EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
UniProt link P06179: Flagellin (Salmonella typhimurium) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Product References
- Deletion of flagellin's hypervariable region abrogates antibody-mediated neutralization and systemic activation of TLR5-dependent immunity: C. Nempont, et al.; J. Immunol. 181, 2036 (2008)
- The Salmonella type-3 secretion system-1 and flagellar motility influence the neutrophil respiratory burst: T.L. Westerman, et al.; PLoS One 13, e0203698 (2018)
- Siglec-14 Enhances NLRP3-Inflammasome Activation in Macrophages: C.M. Tsai, et al.; J. Innate Immun. 12, 333 (2019)
- hGBP1 Coordinates Chlamydia Restriction and Inflammasome Activation through Sequential GTP Hydrolysis: A. Xavier, et al.; Cell Rep. 31, 107667 (2020)
- LRR-protein RNH1 dampens the inflammasome activation and is associated with COVID-19 severity: G. Bombaci, et al.; Life Sci. Alliance 5, e202101226 (2022)
- Study of Agonists of TLRs as Vaccine Adjuvants: F. Mancini, et al.; Methods Mol. Biol. 2700, 249 (2023)
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