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AdipoGen Life Sciences
LAG-3 (human):Fc (human) (rec.)
395
CHF
CHF 395.00
In stock
AG-40B-0031-C05050 µgCHF 395.00
Figure 1: Binding of LAG-3 (human):Fc (human) (rec.) (Prod. No. AG-40B-0031) on dendritic and B cells. Method: Monocyte-derived immature DC (iDC), DC matured for 48h with LPS (mDC) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10µg/ml) or LAG-3 (human):Fc (human) (rec.) (10µg/ml) for 30 min. at 4°C in PBS/1% BSA. The cells are then stained for 30 min. at 4°C with FITC-labeled goat anti-human Fc antibodies.
Figure 2: LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation.
Method: Immature DCs derived from human monocytes, resuspended at 1x106 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (Prod. No. AG-40B-0031) (10µg/ml), LPS (positive control, 5µg/ml) or human IgG1 (isotype-matched negative control, 10µg/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
Method: Immature DCs derived from human monocytes, resuspended at 1x106 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (Prod. No. AG-40B-0031) (10µg/ml), LPS (positive control, 5µg/ml) or human IgG1 (isotype-matched negative control, 10µg/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
| Product Details | |
|---|---|
| Synonyms | Lymphocyte Activation Gene-3; FDC Protein; CD223 |
| Product Type | Protein |
| Properties | |
| Source/Host | CHO cells |
| Sequence |
The sequence coding for the 4 extracellular Ig-like domains of human LAG-3 (D1-D4) is fused to the Fc portion of human IgG1. |
| Crossreactivity |
Human Monkey Mouse |
| Specificity |
Binds to human, mouse and monkey MHC class II. |
| Biological Activity |
Inhibits binding of MAb to LAG-3 (human) to LAG-3. Induces maturation of human dendritic cells. |
| MW | ~80kDa (SDS-PAGE) |
| Purity | ≥99% (SDS-PAGE) |
| Endotoxin Content | <0.001EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.5 mg/ml |
| Formulation | Liquid. In PBS containing 25mM arginine and 0.02% polysorbate 80. |
| Protein Negative Control | |
| Other Product Data |
Application: Flow Cytometry: Detection of MHC class II molecules in combination with fluorescently labeled antibodies to IgG1. Product contains arginine for optimized protein stability. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. Contains arginine for optimized protein stability. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Lymphocyte activation gene 3 (LAG-3; CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.
Product References
- CD4/major histocompatibility complex class II interaction analyzed with CD4- and lymphocyte activation gene-3 (LAG-3)-Ig fusion proteins: B. Huard, et al.; Eur. J. Immunol. 25, 2718 (1995)
- T cell major histocompatibility complex class II molecules down-regulate CD4+ T cell clone responses following LAG-3 binding: B. Huard, et al.; Eur. J. Immunol. 26, 1180 (1996)
- Maturation and activation of dendritic cells induced by lymphocyte activation gene-3 (CD223): S. Andreae, et al.; J. Immunol. 168, 3874 (2002)
- LAG-3 (CD223) reduces macrophage and dendritic cell differentiation from monocyte precursors: S. Buisson & F. Triebel; Immunology 114, 369 (2005)
- A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006)
- A soluble form of lymphocyte activation gene-3 (IMP321) induces activation of a large range of human effector cytotoxic cells: C. Brignone, et al.; J. Immunol. 179, 4202 (2007)
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