Fc (human):Dectin-1 (mouse) (rec.)
|Synonyms||C-type Lectin Domain Family 7 Member A; Β-Glucan Receptor; Dendritic Cell-associated C-type Lectin 1; C-type Lectin Superfamily Member 12; DC-associated C-type Lectin 1|
|Source/Host||HEK 293 cells|
|Sequence||Mouse dectin-1 (aa 72-244) is fused at the C-terminus to the Fc portion of human IgG1.|
|Endotoxin Content||<0.01EU/μg purified protein (LAL test; Lonza).|
|Concentration||1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 50μl sterile water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||UniProt link Q6QLQ4: Dectin-1 (mouse)|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.
|Use/Stability||Stable for at least 6 months after receipt when stored at -20°C.|
|Product Specification Sheet|
Dectin-1 and dectin-2 are type II transmembrane proteins of the C-type lectin family. They contain single carbohydrate recognition domains (CRDs) in their extracellular region. Dectin-1 and dectin-2 are expressed in macrophages and dendritic cells. Dectin-1 recognizes β-glucans with its CRD and signals through its immunoreceptor tyrosine-based activation motif (ITAM)-like motif in the cytoplasmic domain, whereas dectin-2 recognizes α-mannans and transduces its signal through association with the ITAM-containing Fc receptor γ-chain. Dectin-1 controls fungal infection by sensing β-glucans and by mounting a Th17-mediated immune responses. Dectin-1 seems to detect also non-fungal ligands such as invertebrate tropomyosin. Dectin-1 by binding to non-fungal ligands controls type 2 immunity in the lungs in response to the dust mite aeroallergen through the regulation of IL-33-driven IL-13 production from innate lymphoid cells.
- Quantitative Analysis of Candida Cell Wall Components by Flow Cytometry with Triple-Fluorescence Staining: M.F. Nogueira, et al.; J. Microbiol. Modern Techn. 2, 2017