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AdipoGen Life Sciences
IL-37 (human) (monomeric):Fc-KIH (human) (rec.)
450
CHF
CHF 450.00
In stock
AG-40B-0221-C01010 µgCHF 450.00
AG-40B-0221-30103 x 10 µgCHF 900.00
AG-40B-0221-C100100 µgCHF 1'900.00
Figure 1: Protein structure of IL-37 (human) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0221).
Figure 2: IL-37 (human) (monomeric):Fc-KIH (human) (rec.) (#AG-40B-0221) inhibits LPS-induced IL-6 expression. Method: Fc-KIH (human) IgG1 Control (rec.) (#AG-35B-0015) (Control) or IL-37 (human) (monomeric):Fc-KIH (human) (rec.) (#AG-40B-0221) (at the indicated concentrations) are added to freshly isolated human peripheral blood mononuclear cells (PBMCs) for 30 minutes before stimulation with LPS (50 pg/mL). After 20 hours incubation, IL-6 is determined by AlphaLISA using PBMC culture supernatants.
| Product Details | |
|---|---|
| Synonyms | InVivoKine™; IL-37 (human) (monomeric):Fc Knobs-into-Holes (human) (rec.); Interleukin-1 Family Member 7; IL-1F7; FIL1 zeta; IL-1X; Interleukin-1 Homolog 4; IL-1H4; Interleukin-1 zeta; IL-1 zeta; IL-1RP1; IL1F7; FIL1Z, IL1H4, IL1RP1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
Human IL-37 (aa 46-218) (Δ5CysS-S and Y85A) (see reference: A. Bujotzek, et al. 2022) is fused at the C-terminus to a Fc (human) IgG1 (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)). |
| Crossreactivity |
Human Mouse |
| Application |
Shown to have anti-inflammatory activity. |
| Specificity |
Works in human and mouse models. |
| MW | ~55kDa and 28kDa (SDS-PAGE); ~90 kDa (Monomer) (SEC) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml |
| Reconstitution | Reconstitute with 100 µl endotoxin-free water. |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control | |
| Other Product Data |
NCBI link NP_055254: IL-37 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
IL-37 (IL-1F7; IL-1H4) is an IL-1 family member that is expressed only in certain types of human organs and cells such as heart, thymus, testis, kidney, mononuclear cells (PBMCs) and dendritic cells. IL-37 is a soluble and secreted cytokine of 218 residues that shares the β-trefoil fold common to IL-1 family cytokines. But IL-37 is an atypical member of the IL-1 family of cytokines as it functions to inhibit many of the major pathways of innate and adaptive immunity and inflammation. It binds to the interleukin-18 receptor (IL-18R) and its co-receptor SIGIRR. IL-37 is secreted as a full-length and as a processed form starting from amino acid Val46 and assembles into an activity-limiting dimer with a dimerization constant in the nanomolar range. IL-37 mutations that lock the cytokine into its monomeric conformation (e.g. Y85A and D73K) are more active at blocking inflammation than wild-type dimer-forming IL-37 in a broad range of in vitro and in vivo activity assays. IL-37 also inhibited Lipopolysaccharide (LPS)-induced immunological reaction and LPS-induced osteoclast formation and bone resorption.
IL-37 (human) (monomeric):Fc-KIH (human) (rec.) (Prod. No. AG-40B-0221) is a new recombinant monomeric IL-37 produced in mammalian cells that has enhanced stability and activity compared to the classical human recombinant IL-37 produced in bacteria. This monomeric IL-37 protein is produced by using two different vectors, one encoding for the IL-37 (monomeric):Fc Knobs sequence (synthesizing a protein of 50kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-37 (human) (monomeric):Fc-KIH (human) (rec.).
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
Product References
- Protein engineering of a stable and potent anti-inflammatory IL-37-Fc fusion with enhanced therapeutic potential: A. Bujotzek, et al.; Cell Chemical Biol. 29, 1 (2022)
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