Fc-KIH (human) IgG1 Control (rec.)

CHF 340.00
In stock
AG-35B-0015-C01010 µgCHF 340.00
AG-35B-0015-30103 x 10 µgCHF 680.00
AG-35B-0015-C100100 µgCHF 1'360.00
More Information
Product Details
Synonyms Fc Knobs-into-Holes (human) IgG1; Immunoglobulin 1 Fragment Constant
Product Type Protein
Properties
Source/Host HEK 293 cells
Sequence

 Fc (human) IgG1 (knobs-into-holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996).

Crossreactivity Human
Mouse
MW ~28kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg purified protein (LAL test).
Concentration After reconstitution:
for 10µg size: 0.1mg/ml
for 100µg size: 1mg/ml
Reconstitution Reconstitute with 100µl endotoxin-free water.
Formulation Lyophilized. Contains PBS.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF

Negative control in experiments with recombinant proteins fused to human IgG1 Fc-KIH (Knobs-into-Holes) (e.g. Prod. No. AG-40B-0221). This protein is not an appropriate negative control for other standard Fc protein constructs.

The protein Fc-KIH (human) IgG1 Control (rec.) is produced by using two different vectors, one encoding for the Fc Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc-KIH (human) IgG1 Control (rec.).

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