Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
Fc InVivoKine™ Human IgG1 Control
As low as
210
CHF
CHF 210.00
In stock
Only %1 left
AG-35B-0015-C01010 µgCHF 210.00
AG-35B-0015-30103 x 10 µgCHF 420.00
AG-35B-0015-C100100 µgCHF 960.00
Figure 1: Fc InVivoKine™ (human) IgG1 Control (#AG-35B-0015) does not bind to the human Fcg Receptor I. Method: Fcg Receptor I (mouse) is coated on an ELISA plate at 1 µg/ml overnight at RT. Fc InVivoKine™ (human) IgG1 Control, a control Fc (silent) InVivoKine™ (human) IgG1 Control (#AG-35B-0018), or a classical Fc (human) IgG1 Control (rec.) (#AG-35B-0007) are added (starting at 4000 ng/ml with a two-fold serial dilution) during one hour and then detected using an anti-human Fc (HRP) antibody. Both control proteins bind to the mouse Fcg Receptor, while the Fc (LALA-PG)-KIH (human) IgG1 Control (rec.) shows no binding as expected with its LALA-PG mutations.
Figure 2: Protein structure of Fc InVivoKine™ (human) IgG1 Control (Prod. No. AG-35B-0015).
Specifications / Handling
| Product Details | |
|---|---|
| Synonyms | Fc KIH (human) IgG1; Immunoglobulin 1 Fragment Constant |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
Fc (human) IgG1 (knobs-into-holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996). |
| Crossreactivity |
Human Mouse |
| MW | ~28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml |
| Reconstitution | Reconstitute with 100µl endotoxin-free water. |
| Formulation | Lyophilized. Contains PBS. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Scientific Background Information
Product Description
Negative control in experiments with recombinant proteins fused to human IgG1 Fc-KIH (Knobs-into-Holes) (e.g. Prod. No. AG-40B-0221). This protein is not an appropriate negative control for other standard Fc protein constructs.
The Fc InVivoKine™ Human IgG1 Control is produced by using two different vectors, one encoding for the Fc Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc InVivoKine™ Human IgG1 Control.
InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.
Related Products
