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AdipoGen Life Sciences
Fc-KIH (human) IgG1 Control (rec.)
340
CHF
CHF 340.00
In stock
AG-35B-0015-C01010 µgCHF 340.00
AG-35B-0015-30103 x 10 µgCHF 680.00
AG-35B-0015-C100100 µgCHF 1'360.00
Figure 1: Protein structure of Fc-KIH (human) IgG1 Control (rec.) (Prod. No. AG-35B-0015).
| Product Details | |
|---|---|
| Synonyms | Fc Knobs-into-Holes (human) IgG1; Immunoglobulin 1 Fragment Constant |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells. Produced using animal component-free medium. |
| Sequence |
Fc (human) IgG1 (knobs-into-holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996). |
| Crossreactivity |
Human Mouse |
| MW | ~28kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 100µg size: 1mg/ml |
| Reconstitution | Reconstitute with 100µl endotoxin-free water. |
| Formulation | Lyophilized. Contains PBS. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Negative control in experiments with recombinant proteins fused to human IgG1 Fc-KIH (Knobs-into-Holes) (e.g. Prod. No. AG-40B-0221). This protein is not an appropriate negative control for other standard Fc protein constructs.
The protein Fc-KIH (human) IgG1 Control (rec.) is produced by using two different vectors, one encoding for the Fc Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc-KIH (human) IgG1 Control (rec.).
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