Fc-KIH (human) IgG1 Control (rec.)
|Synonyms||Fc Knobs-into-Holes (human) IgG1; Immunoglobulin 1 Fragment Constant|
|Source/Host||HEK 293 cells|
Fc (human) IgG1 (knobs-into-holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996).
|Endotoxin Content||<0.01EU/μg purified protein (LAL test).|
for 10µg size: 0.1mg/ml
for 100µg size: 1mg/ml
|Reconstitution||Reconstitute with 100µl endotoxin-free water.|
|Formulation||Lyophilized. Contains PBS.|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
PBS containing at least 0.1% BSA should be used for further dilutions.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
Negative control in experiments with recombinant proteins fused to human IgG1 Fc-KIH (Knobs-into-Holes) (e.g. Prod. No. AG-40B-0221). This protein is not an appropriate negative control for other standard Fc protein constructs.
The protein Fc-KIH (human) IgG1 Control (rec.) is produced by using two different vectors, one encoding for the Fc Knobs sequence with a small linker (synthesizing a protein of 29kDa) and one encoding for the Fc Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc-KIH (human) IgG1 Control (rec.).