CD152 [CTLA-4] (human) (rec.) (His)
Method: CD80 (human): Fc (mouse) (rec.) (Prod. No. CHI-HF-211CD80) is coated on an ELISA plate at 1 μg/ml overnight at 4°C. CD152 [CTLA-4] (human) (rec.) (His) (Prod. No. AG-40B-0228) (red line) or a control protein (His) (green line) is incubated (starting at a concentration of 8,000 ng/ml with a two-fold serial dilution) during one hour at RT and the interaction is then detected using an anti-His-(HRP).
|Synonyms||Cytotoxic T-lymphocyte-associated Antigen 4; Cytotoxic T-lymphocyte Protein 4|
|Source/Host||HEK 293 cells|
Human CD152 [CTLA-4] (aa 36-161) is fused at the C-terminus to a His-tag.
Binds to human CD80.
|Endotoxin Content||<0.01EU/μg purified protein (LAL test).|
|Concentration||1mg/ml after reconstitution.|
|Reconstitution||Reconstitute with 50µl endotoxin-free water.|
|Formulation||Lyophilized. Contains PBS.|
|Other Product Data||
UniProt link P16410: CD152 /CTLA4 (human) Protein
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Centrifuge lyophilized vial before opening and reconstitution.
Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.
|Product Specification Sheet|
CD152 (Cytotoxic T-lymphocyte-associated protein 4; CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T cell homeostasis and self-tolerance.
While CD28 promotes T cell activation and proliferation, CTLA-4 is reported to dampen T cell responses through a variety of mechanisms. Prior to activation, conventional T cells (Tconv) express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface. In Tregs on the other hand, transmembrane CTLA-4 is constitutively expressed and plays an important role in Treg homeostasis and function. In general, T cell CTLA-4 is largely constrained to intracellular expression although some surface expression may be detectable owing to the rapid, continuous shuttling of CTLA-4 between intracellular compartments and the plasma membrane.
Recently, CTLA-4 has been studied in dendritic cells and tumors, showing that CTLA-4 plays nonredundant and critical roles in thymic development, T cell priming, peripheral tolerance, and a variety of other critical immunoregulatory functions as an immune checkpoint in Immuno-oncology research.