IFN-γ (mouse):Fc (silent) InVivoKine™

Specifications / Handling

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Product Details
Synonyms	Interferon γ (mouse):Fc (LALA-PG) KIH (human) (rec.); Ifng; IFNG
Product Type	Protein
Properties
Source/Host	HEK 293 cells. Produced using animal component-free medium.
Sequence	Extracellular domain of mouse IFN-γ (aa 23-155) is fused at the C-terminus to the Fc portion of human IgG1 (LALA-PG) (Knobs-into-Holes technology) (see reference: J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)).
Crossreactivity	Mouse
Specificity	The Fc contains the mutations LALA-PG that abolish the interaction between the Fc and FcγRs and therefore Fc undesirable effects. The Fc (LALA-PG) is named Fc (Silent).
MW	~60kDa and 28kDa (SDS-PAGE)
Purity	≥95% (SDS-PAGE)
Endotoxin Content	<0.01 EU/μg protein (LAL test).
Concentration	After reconstitution: 1mg/ml
Reconstitution	Reconstitute with 50μl endotoxin-free water.
Accession Number	P01580
Formulation	Lyophilized. Contains PBS.
Protein Negative Control	Fc (silent) InVivoKine™ (human) IgG1 Control (Prod. No. AG-35B-0018)
Other Product Data	Uniprot link P01580: IFN-gamma (mouse)
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions.
Use/Stability	Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

Interferons (IFNs) are pleiotropic cytokines with antiviral, antitumor and immunomodulatory properties, being central coordinators of the immune response. Three major types of IFNs (type I, II and III) are distinguished by their sequence identity, genetic loci, cell of origin, nature, and distribution of their receptors and resulting stimuli. Interferon-gamma (IFN-γ) is the only member of the type II Interferon family. It is a homodimer formed by the non-covalent association of two 17kDa polypeptide subunits. Interferon-gamma (IFN-γ) is a pleiotropic molecule with associated antiproliferative, pro-apoptotic and antitumor mechanisms. Interferon-gamma is secreted predominantly by activated lymphocytes such as CD4 T helper type 1 (Th1) cells, CD8 cytotoxic T cells, γδ T cells and natural killer (NK) cells. Interferon-gamma (IFN-γ) signaling is necessary for the proinflammatory activation of macrophages. IFN-gamma promotes host immune response to microorganisms and plays a pivotal role in cancer immune surveillance, stimulating antitumor immunity and promoting tumor recognition and elimination. In the brain, IFN-γ has a unique role in microglial activation. Priming microglia by IFN-γ results in proliferation (microgliosis) and enhanced synapse elimination.

The IFN-γ (mouse):Fc (silent) InVivoKine™ is produced by using two different vectors, one encoding for the IFN-γ (mouse):Fc (LALA-PG) Knobs sequence (synthesizing a protein of 60kDa) and one encoding for the Fc (LALA-PG) Holes sequence (synthesizing a protein of 28kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology; J.B. Ridgway, et al.; Protein Eng. 9, 617 (1996)) required for dimerization of the Fc moieties and for secretion of the final protein IFN-γ (mouse):Fc (silent) InVivoKine™. This Fc-KIH format allows our IFN-γ mouse protein to form a dimer that is the most active structure. The LALA-PG mutations inhibit binding to FcγRs and C1q while FcRn binding and Fc stability remain unaffected.

InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research, developed and manufactured by AdipoGen Life Sciences.

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