NF-κB (p65) (human) (rec.) (His) (highly active)

CHF 390.00
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AG-40T-0020-C0022 µgCHF 390.00
 
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Product Details
Synonyms Nuclear Factor NF-κB p65 Subunit; Transcription Factor p65; RELA
Product Type Protein
Properties
Source/Host Sf21 cells
Sequence Human NF-κB (p65) is fused to a His-tag.
Crossreactivity Human
Biological Activity ~1ng is required for high mobility shift assay.
~5ng are required for reconstituted transcription assays.
Purity ≥95% (SDS-PAGE)
Concentration Lot dependent (0.2-1.5mg/ml)
Formulation Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50mM imidazole and 10% glycerol.
Other Product Data UniProt ID Q04206: NF-κB (human) (p65 subunit)
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -80°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
NF-κB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at κB sites in the DNA of their target genes and the individual dimers have distinct preferences for different κB sites that they can bind with distinguishable affinity and specificity.
Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κB inhibitor (I-κB) family. In a conventional activation pathway, I-κB is phosphorylated by I-κB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κB complex which translocates to the nucleus. NF-κB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-κB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-κB complex.
Product References
  1. Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003)
  2. Arginine methyltransferase CARM1 is a promoter-specific regulator of NF-kappaB-dependent gene expression: M. Covic, et al.; EMBO J. 24, 85 (2005)
  3. Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005)
  4. MYBBP1a is a novel repressor of NF-κB: H. R. Owen, et al.; J. Mol. Biol. 366, 725 (2007)
  5. Oxidized guanine base lesions function in 8-oxoguanine DNA glycosylase-1-mediated epigenetic regulation of Nuclear Factor κB-driven gene expression: L. Pan, et al.; J. Biol. Chem. 291, 25553 (2016)
  6. OGG1-DNA interactions facilitate NF-κB binding to DNA targets: L. Pan, et al.; Sci. Rep. 7, 43297 (2017)
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