NF-κB (p50) (human) (rec.) (His) (highly active)
AG-40T-0021-C0022 µgCHF 450.00
|Synonyms||Nuclear Factor NF-κB p50 Subunit|
|Sequence||Human NF-κB (p50) is fused to a His-tag.|
~1ng is required for high mobility shift assay.
~5ng are required for reconstituted transcription assays.
|Concentration||Lot dependent (approx. 0.2mg/ml)|
|Formulation||Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.|
|Other Product Data||UniProt ID P19838: NF-κB (human) (p50 subunit)|
|Shipping and Handling|
|Short Term Storage||-20°C|
|Long Term Storage||-80°C|
After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.
|Use/Stability||Stable for at least 6 months after receipt when stored at -80°C.|
|Product Specification Sheet|
NF-κB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at κB sites in the DNA of their target genes and the individual dimers have distinct preferences for different κB sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κB inhibitor (I-κB) family. In a conventional activation pathway, I-κB is phosphorylated by I-κB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κB complex which translocates to the nucleus. NF-κB heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-κB p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3.
- Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003)
- Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005)
- Oxidized guanine base lesions function in 8-oxoguanine DNA glycosylase-1-mediated epigenetic regulation of Nuclear Factor κB-driven gene expression: L. Pan, et al.; J. Biol. Chem. 291, 25553 (2016)
- OGG1-DNA interactions facilitate NF-κB binding to DNA targets: L. Pan, et al.; Sci. Rep. 7, 43297 (2017)