AdipoGen Life Sciences

TDO (Anopheles gambiae) (rec.) (His)

CHF 590.00
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AG-40T-0101-C05050 µgCHF 590.00
 
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Product Details
Synonyms TPH2; TRPO; Tryptophan 2,3-dioxygenase; Tryptophan Oxygenase; Tryptophan Pyrrolase; Tryptophanase
Product Type Protein
Properties
Source/Host E. coli
Sequence Full-length TDO (Anopheles gambiae) is fused at the N-terminus to a His-tag.
Application Can be used to test potential kynurenine pathway inhibitors in the mosquito, by continuously monitoring the rate of NFK formation at 321nm (ε=3152/M cm) in in vitro spectrophotometric assays.
Biological Activity Specific activity towards L-Trp: 87.0μmol/hr per mg. TDO catalyzes the oxidative opening of the L-tryptophan indole moiety to yield N-formylkynurenine (NFK).
MW ~47.9kDa
Purity ≥98% (SDS-PAGE)
Endotoxin Content N/A.
Concentration 0.5mg/ml (Lot specific)
Formulation Liquid. In 50mM sodium phosphate pH 8.0, containing 0.15M sodium chloride, 0.2M imidazole and 10% glycerol.
Other Product Data UniProt link O77457: TDO (Anopheles gambiae)
Shipping and Handling
Shipping DRY ICE
Short Term Storage -20°C
Long Term Storage -80°C
Handling Advice After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -80°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description
Tryptophan 2,3-dioxygenase (TDO) is a heme-containing dioxygenase catalyzing the addition of molecular oxygen across the 2,3-double bond of the indole ring of tryptophan to form N-formylkynurenine (NFK). In Anopheles gambiae, TDO is the only enzyme able to catalyze the first and rate-limiting step in L-Trp catabolism through the kynurenine pathway. Tryptophan is an essential amino acid for protein synthesis and also the precursor for production of a number of neurotransmitters, such as serotonin and melatonin; in mosquitoes, the kynurenine pathway is essential for eye pigmentation. Conceivably, the tryptophan-using pathways should be regulated in a coordinated manner in mosquitoes as well as in other species and TDO activation/inactivation processes could play an essential role in these phenomena.
Product References
  1. Anopheles gambiae tryptophan 2,3-dioxygenase expressed in Escherichia coli: A. Paglino, et al.; Insect Biochem. Mol. Biol. 38, 871 (2008)
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