NAD Kinase (B. subtilis) (recombinant protein)

Specifications / Handling

More Information
Product Details
Synonyms	Probable Inorganic Polyphosphate/ATP-NAD Kinase; Poly(P)/ATP NAD Kinase; ppnK; yjbN; EC 2.7.1.23
Product Type	Protein
Properties
Source/Host	E. coli
Sequence	Full-length NAD kinase (Bacillus subtilis).
Biological Activity	~2.6 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
MW	~30.0kDa
Purity	≥98% (SDS-PAGE)
Endotoxin Content	N/A
Concentration	Lot specific
Formulation	Liquid. In 50mM TRIS-HCl, pH 7.0 containing 150mM sodium chloride, 1mM EDTA and 1mM dithiothreitol.
Other Product Data	UniProt link O31612: NAD Kinase (B. subtilis)
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-20°C
Long Term Storage	-80°C
Handling Advice	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -80°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD⁺ to generate NADP⁺, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP⁺ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP⁺ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus.
Catalytic activity: ATP + NAD⁺ = ADP + NADP⁺

Product References

Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid: S. Garavaglia, et al.; J. Bacteriol. 185, 4844 (2003)

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NAD Kinase (B. subtilis) (rec.)