AdipoGen Life Sciences
| Product Details | |
|---|---|
| Synonyms | NADK; Poly(P)/ATP NAD Kinase; EC 2.7.1.23 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human NAD kinase (aa 64-446) is fused at the N-terminus to a His-tag. |
| Crossreactivity | Human |
| Biological Activity | ≥2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NADP per min. |
| MW | ~42kDa (monomer) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | N/A. Free of any nucleotide converting side-activities. |
| Concentration | 1mg/ml |
| Reconstitution | Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH7.5, 150mM sodium chloride and 1mM DTT. |
| Formulation | Liquid. Ammonium sulphate suspension. |
| Other Product Data | UniProt link O95544: NAD kinase (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
![Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10μl per 50μl reaction mix were applied in the reaction mix (20](https://adipogen.com/media/catalog/product/cache/60eb5af712bc93baae8d55513bd31b01/a/g/ag-40a-0018_h-nampt_visfatin_-his-enzymatic-activity.png)
![Measurement of NAMPT enzymatic activity was performed as described previously [G.C. Elliott, et al.; Anal Biochem 107, 199 (1980)]. The recombinant Nampt was diluted in assay buffer and 10μl per 50μl reaction mix were applied in the reaction mix (20](https://adipogen.com/media/catalog/product/cache/60eb5af712bc93baae8d55513bd31b01/a/g/ag-40a-0018aa_h-nampt_visfatin_-his-enzymatic-activity.png)