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AdipoGen Life Sciences
NAD Kinase (catalytic domain) (human) (rec.) (His) (highly active)
540
CHF
CHF 540.00
In stock
AG-40T-0090-C05050 µgCHF 540.00
| Product Details | |
|---|---|
| Synonyms | NADK; Poly(P)/ATP NAD Kinase; EC 2.7.1.23 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human NAD kinase (aa 64-446) is fused at the N-terminus to a His-tag. |
| Crossreactivity | Human |
| Biological Activity | ≥2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NADP per min. |
| MW | ~42kDa (monomer) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | N/A. Free of any nucleotide converting side-activities. |
| Concentration | 1mg/ml |
| Reconstitution | Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH7.5, 150mM sodium chloride and 1mM DTT. |
| Formulation | Liquid. Ammonium sulphate suspension. |
| Other Product Data | UniProt link O95544: NAD kinase (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis.
Product References
- Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001)
- NADPH regulates human NAD kinase, a NADP+-biosynthetic enzyme: K. Ohashi, et al.; Mol. Cell Biochem. 355, 57 (2011)
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