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AdipoGen Life Sciences
NMNAT3 (human) (rec.) (His) (highly active)
260
CHF
CHF 260.00
In stock
AG-40T-0093-C01010 µgCHF 260.00
AG-40T-0093-C05050 µgCHF 600.00
| Product Details | |
|---|---|
| Synonyms | Nicotinamide Mononucleotide Adenylyltransferase 3; NMN Adenylyltransferase 3; NaMN Adenylyltransferase 3; EC 2.7.7.18; EC 2.7.7.1 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag. |
| Crossreactivity | Human |
| Application | Well suited for the synthesis of NAD analogs due to lower substrate selectivity compared to NMNAT1 (human) (rec.) (His) (highly active). For NAD synthesis use NMNAT1 (human) (rec.) (His) (highly active) (AG-40T-0092). |
| Biological Activity | ≥2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NAD+ per min. |
| MW | ~27kDa (monomer) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | N/A |
| Concentration | 1mg/ml |
| Formulation | Liquid. In 50mM TRIS-HCl, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol. |
| Other Product Data | UniProt link Q96T66: NMNAT3 (human) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Mitochondrial NMNAT isoform. Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NAADP+. Protects against axonal degeneration following injury.
Product References
- Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis: X. Zhang, et al.; J. Biol. Chem. 278, 13503 (2003)
- Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005)
- The NMN/NaMN adenylyltransferase (NMNAT) protein family: C. Lau, et al.; Front. Biosci. 14, 410 (2009)
- Isoform-specific targeting and interaction domains in human nicotinamide mononucleotide adenylyltransferases: C. Lau, et al.; J. Biol. Chem. 285, 18868 (2010)
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