NAD Synthetase (B. subtilis) (recombinant protein) (recombinant protein)

Specifications / Handling

More Information
Product Details
Synonyms	NH(3)-dependent NAD(+) Synthetase; General Stress Protein 38; GSP38; Spore Outgrowth Factor B; EC6.3.1.5
Product Type	Protein
Properties
Source/Host	E. coli
Sequence	Full-length NAD synthetase (Bacillus subtilis).
Biological Activity	~0.3 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol NAD per min.
MW	~30.4kDa
Purity	≥98% (SDS-PAGE)
Endotoxin Content	N/A
Concentration	0.5mg/ml (Lot specific)
Formulation	Liquid. In 50mM TRIS-HCl, pH 8, containing 75mM sodium chloride, 5% glycerol and 5mM DTT.
Other Product Data	UniProt link P08164: NAD Synthetase (B. subtilis)
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-20°C
Long Term Storage	-80°C
Handling Advice	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -80°C.
Documents
Protocols	Download PDF
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD⁺, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore.
Catalytic activity: ATP + deamido-NAD⁺ + NH3 = AMP + diphosphate + NAD⁺

Product References

Crystal structure of NH3-dependent NAD+ synthetase from Bacillus subtilis: M. Rizzi, et al.; EMBO J. 15, 5125 (1996)

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NAD Synthetase (B. subtilis) (rec.)