Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
NAD Nucleotidase (H. influenzae) (rec.)
590
CHF
CHF 590.00
In stock
AG-40T-0110-C05050 µgCHF 590.00
| Product Details | |
|---|---|
| Synonyms | NadN; NAD Pyrophosphatase/5'-nucleotidase |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence | Full-length NAD 5’-nucleotidase (Haemophilus influenzae) without the signal peptide corresponding to the first 26 aa. |
| Biological Activity |
With AMP: 856.4 nmol of Ade/min/mg of protein With NMN: 110,3 nmol of NR/min/mg of protein With NAD: 5.4 nmol of Ade/min per mg of protein | 5.3 nmol of NR/min/mg of protein |
| MW | ~63.0kDa |
| Purity | ≥98% (SDS-PAGE) |
| Endotoxin Content | N/A |
| Concentration | 0.5mg/ml (Lot specific) |
| Formulation | Liquid. In 5mM KH2PO4, pH 6.8 containing 100mM TRIS-HCl (pH 8.5) and 10mM MgCl2. |
| Other Product Data | UniProt link Q4QNY4: NAD Nucleotidase (H. influenza) |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice |
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -80°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
NadN (NAD nucleotidase) is a periplasmic enzyme from Haemophilus influenzae, a major pathogen of the respiratory tract in humans that has developed the capability to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degrading NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside).
Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi
Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi
Product References
- The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism: S. Garavaglia, et al.; Biochem. J. 441, 131 (2012)
Related Products
