AdipoGen Life Sciences

X-Gal

CHF 30.00

In stock

AG-CC1-0003-M05050 mgCHF 30.00

AG-CC1-0003-M250250 mgCHF 40.00

AG-CC1-0003-G0011 gCHF 55.00

AG-CC1-0003-G0055 gCHF 180.00

More Information
Product Details
Synonyms	5-Bromo-4-chloro-3-indolyl β-D-galactoside
Product Type	Chemical
Properties
Formula	C₁₄H₁₅BrClNO₆
MW	408.6
CAS	7240-90-6
Source/Host Chemicals	Synthetic
Purity Chemicals	≥99% (HPLC)
Appearance	White to off-white powder. Clear, colourless to light yellow in solution.
Solubility	Soluble in dimethyl formamide.
Identity	Determined by IR.
Other Product Data	Optical Rotation [α]D20 (c=1,DMF:water, 1:1): -61.5° ± 1.0° Water Content (Karl Fischer): max 1%.
InChi Key	OPIFSICVWOWJMJ-LMNFNBGQSA-N
Smiles	OCC1O[C@@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)C(O)[C@H](O)[C@H]1O
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use/Stability	Stable for at least 3 years after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Description

Molecular biology reagent.
Chromogenic substrate for β-galactosidase.
Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the β-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening.
Distinguishes between recombinant and non-recombinant plasmids carrying the β-galactosidase gene.
Used in two-hybrid analysis.
X-Gal is used to determine E. coli and coliform content in drinking water samples.

Product References

Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay: H. Arakawa, et al.; Anal. Biochem. 199, 238 (1991)
In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity: D.J. Weiss, et al.; Hum. Gene Ther. 8, 1545 (1997)
A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins: K.H. Engler, et al.; J. Microbiol. Methods 35, 207 (1999)
Molecular Cloning: A Laboratory Manual 3rd Edition: J. Sambrook & D. Russell; Cold Spring Harbor Laboratory Press (2001)
Effects of tissue pressure on transgene expression characteristics via renal local administration routes from ureter or renal artery in the rat kidney: N. Oyama, et al.; MDPI Pharmaceutics 12, 114 (2020)

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