AdipoGen Life Sciences

X-Gal

As low as CHF 35.00

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AG-CC1-0003-M05050 mgCHF 35.00

AG-CC1-0003-M250250 mgCHF 45.00

AG-CC1-0003-G0011 gCHF 60.00

AG-CC1-0003-G0055 gCHF 190.00

AG-CC1-0003-G02525 gCHF 760.00

Specifications / Handling

More Information
Product Details
Synonyms	5-Bromo-4-chloro-3-indolyl β-D-galactoside
Product Type	Chemical
Properties
Formula	C₁₄H₁₅BrClNO₆
MW	408.6
CAS	7240-90-6
Source/Host Chemicals	Synthetic
Purity Chemicals	≥99% (HPLC)
Appearance	White to off-white powder. Clear, colourless to light yellow in solution.
Solubility	Soluble in dimethyl formamide.
Identity	Determined by ¹H-NMR.
Other Product Data	Optical Rotation [α]D20 (c=1,DMF:water, 1:1): -61.5° ± 1.0° Water Content (Karl Fischer): max 1%.
InChi Key	OPIFSICVWOWJMJ-LMNFNBGQSA-N
Smiles	OCC1O[C@@H](OC2=CNC3=C2C(Cl)=C(Br)C=C3)C(O)[C@H](O)[C@H]1O
Shipping and Handling
Shipping	AMBIENT
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	Protect from light and moisture.
Use/Stability	Stable for at least 3 years after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product Description

Molecular biology reagent.
Chromogenic substrate for β-galactosidase.
Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the β-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening.
Distinguishes between recombinant and non-recombinant plasmids carrying the β-galactosidase gene.
Used in two-hybrid analysis.
X-Gal is used to determine E. coli and coliform content in drinking water samples.

Product-specific References

Chemiluminescent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay: H. Arakawa, et al.; Anal. Biochem. 199, 238 (1991)
In situ histochemical detection of beta-galactosidase activity in lung: assessment of X-Gal reagent in distinguishing lacZ gene expression and endogenous beta-galactosidase activity: D.J. Weiss, et al.; Hum. Gene Ther. 8, 1545 (1997)
A novel colorimetric yeast bioassay for detecting trichothecene mycotoxins: K.H. Engler, et al.; J. Microbiol. Methods 35, 207 (1999)
Molecular Cloning: A Laboratory Manual 3rd Edition: J. Sambrook & D. Russell; Cold Spring Harbor Laboratory Press (2001)
Effects of tissue pressure on transgene expression characteristics via renal local administration routes from ureter or renal artery in the rat kidney: N. Oyama, et al.; MDPI Pharmaceutics 12, 114 (2020)

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