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AdipoGen Life Sciences
7-Azido-4-methylcoumarin
45
CHF
CHF 45.00
In stock
AG-CR1-3525-M0011 mgCHF 45.00
AG-CR1-3525-M0055 mgCHF 90.00
| Product Details | |
|---|---|
| Synonyms | AzMC |
| Product Type | Chemical |
| Properties | |
| Formula |
C10H7N3O2 |
| MW | 201.2 |
| CAS | 95633-27-5 |
| Purity Chemicals | ≥98% (NMR) |
| Appearance | White to yellow solid. |
| Solubility | Soluble in DMSO. |
| Identity | Determined by 1H-NMR and MS. |
| InChi Key | HEKDKVLIMUWRRZ-UHFFFAOYSA-N |
| Smiles | CC1=CC(=O)OC2=CC(=CC=C12)N=[N+]=[N-] |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light. |
| Use/Stability |
Stable for at least 2 years after receipt when stored at -20°C. Store solutions at -20°C in the dark. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
- Highly sensitive and selective fluorogenic H2S probe [1-3]. The aromatic azide moiety of AzMC is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with λex = 365 nm and λem = 450 nm.
- Photoaffinity labeling probe for the substrate binding site of human sulfotransferase 1A1 (SULT1A1).
- Probe to monitor the enzymatic production of H2S in vitro and to visualize H2S in living cells [2, 3].
- Tool for monitoring the activity of pyridoxal-5'-phosphate (PLP)-dependent enzymes (e.g. cystathionine β-synthase (CBS), cystathionine γ-lyase (CGL) and tryptophan synthase (TS)) [2].
- Tool to identify novel cystathionine β-synthase (CBS) inhibitors and activators [2].
- Suitable for high-throughput screening [2].
- Caution: Use of this product with DTT, TCEP and/or biological thiols at concentrations of >25 mM should be avoided for maximum efficiency [2].
Product References
- Photoaffinity labeling probe for the substrate binding site of human phenol sulfotransferase (SULT1A1): 7-azido-4-methylcoumarin: G. Chen, et al.; Protein Sci. 8, 2151 (1999)
- Identification of cystathionine β-synthase inhibitors using a hydrogen sulfide selective probe: M.K. Thorson, et al.; Angew. Chem. Int. Ed. Engl. 52, 4641 (2013)
- Fluorescent probe for highly selective and sensitive detection of hydrogen sulfide in living cells and cardiac tissues: B. Chen, et al.; Analyst. 138, 946 (2013)
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