Kdo2-Lipid A (ready-to-use)

CHF 170.00
In stock
AG-CU1-0001-M0011 mgCHF 170.00
More Information
Product Details
Synonyms (3-Deoxy-D-manno-octulosonic acid)2-Lipid A
Product Type Chemical
Properties
Formula

C110H198N2Na4O39P2

MW 2326.7
CAS 123621-04-5 (free base)
Source/Host Chemicals Isolated and purified from E. coli K12 heptose-deficient strain WBB06 (Re mutant).
Purity Chemicals Absence of detectable protein or DNA contaminants with agonistic TLR activity.
Appearance Clear, colorless solution.
Formulation Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
Concentration 0.5 mg/ml. Vial contains 2 ml.
Other Product Data

Strong activator (<10ng/ml) of toll-like receptor 4 (TLR4). Does not activate TLR2 or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice. No further re-extraction required.

Can be used for priming of the inflammasome at a concentration of 50-100ng/ml.

Isolated and purified from E. coli K12 heptose-deficient strain WBB06 (Re mutant) [1] by a modification of the PCP extraction method [2,3], converted to the uniform sodium salt form [4] and dissolved in sterile pyrogen-free double distilled water.

Lit: [1] Deletion of the heptosyltransferase genes rfaC and rfaF in Escherichia coli K-12 results in a Re-type lipopolysaccharide with a high degree of 2-aminoethanol phosphate substitution: W. Brabetz, et al.; Eur. J. Biochem. 247, 716 (1997) / [2] A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969) / [3] Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Meth. Carbohydrate Chem. 9, 11 (1993) / [4] Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Lüderitz; Eur. J. Biochem. 54, 603 (1975)   
InChi Key HBSUHPVFGADBOQ-USMMBHRMSA-N
Shipping and Handling
Shipping AMBIENT
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Keep sterile. Do not ingest. Wear gloves and mask. Avoid contact through all modes of exposure. Avoid inhalation and prevent from entering the bloodstream. Use must be restricted to qualified personnel.
Use/Stability Stable for at least 2 years after receipt when stored at +4°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
  • Defined substructure of the Re mutant of lipopolysaccharide (LPS) [1].
  • Endotoxin activity equal to Re LPS [1].
  • Strong activator (<10ng/ml) of macrophages via toll-like receptor 4 (TLR4) [1, 2, 3, 4, 9].
  • Does not activate TLR2 [5] or other TLRs as determined with splenocytes and macrophages from TLR4 deficient mice by IL-6 ELISA [1,4].
  • Facilitates the structural analysis of its complexes with signaling receptors, such as TLR4/MD2 [1,2] and CD14 [7].
  • Kdo2-Lipid A was used in a recent animal atherosclerosis model [6].
  • Induces sphingolipid biosynthesis, which is essential for induction of autophagy [8].
Product References
  1. Kdo2-Lipid A of Escherichia coli, a defined endotoxin that activates macrophages via TLR-4: C.R. Raetz, et al.; J. Lipid Res. 47, 1097 (2006)
  2. Aggregation behavior of an ultra-pure lipopolysaccharide that stimulates TLR-4 receptors: H. Sasaki & S.H. White; Biophys. J. 95, 986 (2008)
  3. TLR-4 mediated group IVA phospholipase A(2) activation is phosphatidic acid phosphohydrolase 1 and protein kinase C dependent: A. Grkovich, et al.; BBA 1791, 975 (2009)
  4. Subcellular organelle lipidomics in TLR 4-activated macrophages: A.Y. Andreyev, et al.; J. Lipid Res. 51, 2785(2010)
  5. Spinal glial TLR4-mediated nociception and production of prostaglandin E and TNF: O. Saito, et al.; Br. J. Pharmacol. 160, 1754 (2010)
  6. Low doses of lipopolysaccharide and minimally oxidized low-density lipoprotein cooperatively activate macrophages via nuclear factor kappab and activator protein-1- possible mechanism for acceleration of atherosclerosis by subclinical endotoxemia: P. Wiesner, et al.; Circ. Res. 107, 56 (2010)
  7. NMR spectral mapping of Lipid A molecular patterns affected by interaction with the innate immune receptor CD14: S. Albright, et al.; BBRC 378, 721 (2009)
  8. Kdo2-lipid A, a TLR4-specific agonist, induces de novo sphingolipid biosynthesis in RAW264.7 macrophages, which is essential for induction of autophagy: K. Sims, et al.; J. Biol. Chem. 285, 28568 (2010)
  9. ATF3 plays a key role in Kdo2-lipid A-induced TLR4-dependent gene expression via NF-?B activation: E.Y. Kim, et al.; PLoS One 5, e14181 (2010)
© 2017 Adipogen Life Sciences. Pictures: © 2012 Martin Oeggerli. All Rights Reserved.