anti-CD44 (human), mAb (BU52) (Fab)

Figure 1: Binding of anti-CD44 Fab + GAM/FITC to human Molt-4 cells. Method: Five x 10^5 cultured human Molt-4 cells were incubated 45 minutes on ice with 80 µl of anti-CD44 Fab at 5 µg/ml. Cells were washed twice and incubated with 2nd reagent goat anti-Mouse IgG/FITC (#ANC-232-011), after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.28 log10 fluorescent units when compared to a Mouse IgG1 Fab (#ANC-278-580).