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Ancell
anti-CD47 (human), mAb (ANC2F6) (FITC)
306
CHF
CHF 306.00
In stock
ANC-198-040120 testsCHF 306.00
Figure 1: 5x10^5 cultured CEM cells were washed and incubated 45 min on ice with 80µl of anti-CD47/FITC at a dilution factor of 1:50 (10µg/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.04 log10 fluorescent units when compared to a Mouse IgG1/FITC isotype control (ANC-278-040). Binding was blocked when cells were pre incubated 10 min with 20µl of 0.5mg/ml anti-CD47 antibody (ANC-198-020).
| Product Details | |
|---|---|
| Synonyms | IAP; Integrin Associated Protein; Leukocyte Surface Antigen CD47 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | ANC2F6 |
| Isotype | Mouse IgG1κ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant human CD47 (extracellular Ig domain). |
| Label/Conjugates | FITC |
| Application |
ELISA Flow Cytometry (1:50) |
| Crossreactivity | Human |
| Specificity |
Binds to an epitope within the extracellular Ig-like domain of human CD47. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A purified. |
| Formulation | 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2%BSA, 0.04% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| Accession Number | Q08722 |
| Declaration | Manufactured by Ancell Corporation. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
Avoid freeze/thaw cycles. Protect from light. |
| Use/Stability | Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
Human CD47 (IAP; Integrin Associated Protein) is a broadly expressed Ig superfamily glycoprotein of about 50kD with a long extracellular Ig like domain, 5 transmembrane domains and an alternatively spliced intracellular C-terminal domain. It interacts with its ligand SIRPα (CD172a; Signal Regulatory Protein) expressed mainly on myeloid cells serving as a negative immune regulator, inhibiting phagocytosis. The CD47-SIRPα axis is being investigated as possible anti-tumor immune intervention point. Blockade of this interaction has shown promise in models of HNSCC, glioma and breast cancer.
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