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BellBrook
DDX3 (human) (rec.) (His) (active) (1000µg)
6041
CHF
CHF 6'041.00
In stock
BBL-2253-M0011 mgCHF 6'041.00
| Product Details | |
|---|---|
| Synonyms | ATP-dependent RNA Helicase DDX3X; DEAD Box Protein 3, X-chromosomal; DDX3X; CAP-Rf; HLP2; EC:3.6.4.13 |
| Product Type | Protein |
| Properties | |
| Source/Host | E. coli |
| Sequence |
Recombinant DEAD-box RNA helicase DDX3 enzyme (aa135-583) fused to a His-tag. |
| Crossreactivity | Human |
| Application |
For use with Transcreener ADP2 assay at a concentration range of 15 - 30 nM. |
| Biological Activity |
The enzyme has been thoroughly validated with Transcreener ADP² Activity Assays. For specific activity please refer to the Certificate of Analysis for each individual enzyme lot. |
| Purity | ≥90% (SDS-PAGE) |
| Accession Number | O00571 |
| Formulation | Liquid. In 50mM Tris-HCl pH 8.0, 150mM NaCl, 10% glycerol buffer. |
| Other Product Data |
Uniprot O00571: DDX3X_HUMAN |
| Shipping and Handling | |
| Shipping | DRY ICE |
| Short Term Storage | -20°C |
| Long Term Storage | -80°C |
| Handling Advice | Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -80°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
DDX3 is a DEAD-box group protein and multifunctional ATP-dependent RNA helicase. It is involved in several cellular activities, including innate immunity, viral reproduction, and tumorigenesis. DDX3 unwinds various forms of dsRNA, both 5' - 3' and 3' - 5'. It requires ATP and Mg²+ to unwind and releases RNA via ATP hydrolysis. DDX3 is involved in transcription regulation, in innate immunity acting as a viral RNA sensor and binds viral RNAs promoting the production of type I interferon (IFN-alpha and IFN-beta). In some viruses, such as HIV, DDX3 enables viral replication, while in others (such as Dengue), it has an anti-viral effect. The RNA helicase has similar effects in some cancer studies, wherein one type of DDX3 is a tumor suppressor, while in another it acts as an enhancer. These qualities make DDX3 an interesting target for treating a variety of diseases. DDX3X has been attributed a role in NLRP3 inflammasome activation. DDX3X binds the central NACHT domain of NLRP3 to facilitate NLRP3 oligomerization and inflammasome assembly, both of which are key to NLRP3 inflammasome activation.
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