SMARCAL1 (human) (rec.) (His) (active) (25µg)

Specifications / Handling

More Information
Product Details
Synonyms	SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily A-like Protein 1; hHARP; HepA-related Protein; Sucrose Nonfermenting Protein 2-like 1
Product Type	Protein
Properties
Source/Host	Baculovirus system
Sequence	Truncated human SMARCAL1 (AA 226-870) protein with an N-terminal His-tag.
Crossreactivity	Human
Application	For use with Transcreener ADP² assay.
Biological Activity	The enzyme has been thoroughly validated with Transcreener ADP² Activity Assays. For specific activity please refer to the Certificate of Analysis for each individual enzyme lot.
MW	~72.8kDa
Purity	≥95% (SDS-PAGE)
Accession Number	Q9NZC9
Formulation	Liquid. 50mM HEPES, 100mM NaCl, 1mM TCEP, 10% Glycerol (pH 7.5).
Other Product Data	Click here for Complete Information from the Original Manufacturer Our product description may differ slightly from the original manufacturer's product datasheet. Uniprot Q9NZC9: SMAL1_HUMAN
Shipping and Handling
Shipping	DRY ICE
Short Term Storage	-20°C
Long Term Storage	-80°C
Handling Advice	Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 6 months after receipt when stored at -80°C.
Documents
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

SMARCAL1 is a ATP-dependent DNA annealing helicase involved in maintaining genome stability. It plays a key role in replication fork repair and restart, helping resolve stalled replication forks to prevent DNA damage and ensure proper DNA replication. SMARCAL1 binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. It rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). It acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. Mutations in SMARCAL1 are associated with Schimke immuno-osseous dysplasia (SIOD), a rare multisystem disorder.

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